Reactivos e instrumentos para inmunología, biología celular y biología molecular.
 

Canvax Biotech


Canvax is a leading manufacturer and supplier of the most innovative solutions, kits and R&D Reagents inside Molecular Biology fields. Since its foundation in 2001, Canvax offers reliable, cost-effective and easy-to-use innovative tools for research.

Based in Córdoba (Spain), since its beginning it has focused on R&D of multiplex high throughput platforms (HTS) for Drug discovery and Diagnostic applied biosensors. Over a decade later, Canvax is a worldwide leading expert in GPCR expression in heterologous cells, with important patents and exclusive know-how. Canvax prides itself to be the first company to get an unprecedented milestone that could revolutionize the Diagnostics sector: Canvax established stable high-level expression of odour GPCRs into heterologous cell lines in 2014.

This key milestone, that could be applied in diagnostic, perfume and cosmetic industry, are being employed to obtain a molecular nose, a sensitive and non-invasive diagnostic molecular device for early detection of cancer, as the nose of trained dogs do. Thanks to this project, Canvax was awarded, in 2013, with the largest Public & Private Contract for Pre competitive Technology Development in Spanish history.

 
> Canvax Biotech > Productos para la investigación > Herramientas de biología molecular > Secuenciación de próxima generación (NGS) > NGS Library preparation > Preparation of RNA libraries for NGS > Preparation of RNA libraries for Illumina NGS > NGS Transcriptome sequencing - Library preparation > Sequencing of mRNA by NGS - Library preparation > Sequencing of mRNA by NGS - Library preparation - Modules
Sequencing of mRNA by NGS - Library preparation - Modules

Sequencing of mRNA by NGS - Library preparation - Modules


Total RNA is composed of large amounts of unwanted transcripts, such as ribosomal RNA (rRNA), which accounts for 80-98% of a total RNA sample, and globin mRNA, which accounts for 35-80% of the mRNA in blood samples. Ribosomal RNA is a type of non-coding RNA that is the main component of ribosomes, essential to all cells. Ribosomal RNA is transcribed from ribosomal DNA (rDNA) and then bound to ribosomal proteins to form small and large ribosomal subunits. rRNA is the physical and mechanical component of the ribosome that forces the transfer RNA (tRNA) and messenger RNA (mRNA) to process and translate them into proteins.
Depletion of ribosomal RNA (rRNA) before RNA sequencing (Seq-RNA) is therefore essential and allows us to focus on the analysis of the portions of interest in the transcriptome. The use of oligo dT primer to capture the polyadenylated 3′ tail of transcripts and isolate the mRNA is common in many RNA sequencing preparations; however, this method does not allow processing of degraded samples where most of the RNA is separated from the 3′ tail, nor does it allow isolation of non-polyadenylated transcripts such as ncRNAs. Ribosomal removal methods address these problems by directly depleting rRNA while leaving other transcripts intact.

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