Reactivos e instrumentos para inmunología, biología celular y biología molecular.
 

Canvax Biotech


Canvax is a leading manufacturer and supplier of the most innovative solutions, kits and R&D Reagents inside Molecular Biology fields. Since its foundation in 2001, Canvax offers reliable, cost-effective and easy-to-use innovative tools for research.

Based in Córdoba (Spain), since its beginning it has focused on R&D of multiplex high throughput platforms (HTS) for Drug discovery and Diagnostic applied biosensors. Over a decade later, Canvax is a worldwide leading expert in GPCR expression in heterologous cells, with important patents and exclusive know-how. Canvax prides itself to be the first company to get an unprecedented milestone that could revolutionize the Diagnostics sector: Canvax established stable high-level expression of odour GPCRs into heterologous cell lines in 2014.

This key milestone, that could be applied in diagnostic, perfume and cosmetic industry, are being employed to obtain a molecular nose, a sensitive and non-invasive diagnostic molecular device for early detection of cancer, as the nose of trained dogs do. Thanks to this project, Canvax was awarded, in 2013, with the largest Public & Private Contract for Pre competitive Technology Development in Spanish history.

 
> Canvax Biotech > Productos para la investigación > Herramientas de biología molecular > PCR products > Quantitative RT-PCR - qRT-PCR > RT-qPCR SYBR Green Mixes > Universal SYBR Green Mixes for RT-qPCR
Universal SYBR Green Mixes for RT-qPCR

Universal SYBR Green Mixes for RT-qPCR


The universal SYBR Green quantitative PCR mixes are made for use with all types of qPCR devices.
Mixes contain SYBR Green mix (Taq polymerase, dNTPs, MgCl2 and SYBR Green) and 2 different ROX dyes: High Rox and Low Rox. Simply add the corresponding dye for the qPCR device you want to use.

The advantage of the universal SYBR Green mixes is therefore to be able to use the same mix for different devices.

Here's how to select the appropriate ROX dye for the qPCR device you are using:

Universal All instruments
Without ROX Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2, Chromo4;
Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s;
Illumina Eco qPCR;
Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000;
Roche Applied Science LightCyclerTM 480;
Thermo Scientific PikoReal Cycler.
Low ROX Applied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000,
MX3005P, MX3000P
High ROX Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast;
StepOne, StepOnePlus

For RT-qPCR applications, there are two approaches: one-step RT-qPCR and two-step RT-qPCR. Each of these methods has its advantages and disadvantages. As its name implies, single-step RT-qPCR combines the synthesis of complementary DNA (cDNA) of the first strand (reverse transcript, RT) and subsequent quantitative PCR in a single reaction tube. The two-step RT-qPCR involves two distinct reactions, starting with first strand cDNA synthesis (reverse transcription-RT) and then amplifying part of the resulting cDNA by quantitative PCR in a separate tube .

RT-PCR
One-Step RT-qPCR mixes - SYBR Green
One-Step RT-qPCR mixes - SYBR Green
Two-Step RT-qPCR mixes - SYBR Green
Two-Step RT-qPCR mixes - SYBR Green

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