Canvax Biotech


Canvax is a leading manufacturer and supplier of the most innovative solutions, kits and R&D Reagents inside Molecular Biology fields. Since its foundation in 2001, Canvax offers reliable, cost-effective and easy-to-use innovative tools for research.

Based in Córdoba (Spain), since its beginning it has focused on R&D of multiplex high throughput platforms (HTS) for Drug discovery and Diagnostic applied biosensors. Over a decade later, Canvax is a worldwide leading expert in GPCR expression in heterologous cells, with important patents and exclusive know-how. Canvax prides itself to be the first company to get an unprecedented milestone that could revolutionize the Diagnostics sector: Canvax established stable high-level expression of odour GPCRs into heterologous cell lines in 2014.

This key milestone, that could be applied in diagnostic, perfume and cosmetic industry, are being employed to obtain a molecular nose, a sensitive and non-invasive diagnostic molecular device for early detection of cancer, as the nose of trained dogs do. Thanks to this project, Canvax was awarded, in 2013, with the largest Public & Private Contract for Pre competitive Technology Development in Spanish history.

 
Two-Step RT-qPCR mixes - SYBR Green

Two-Step RT-qPCR mixes - SYBR Green


The two-step RT-qPCR involves two distinct reactions, starting with first strand cDNA synthesis (reverse transcription-RT) and then amplifying part of the resulting cDNA by quantitative PCR in a separate tube . Therefore, two-step RT-qPCR is useful for detecting multiple genes in a single RNA sample. The separation of the RT and quantitative PCR reactions makes it possible to optimize the reaction conditions for each step as well as the flexibility with priming by reverse transcription (oligo (dT) primers, random hexamers or gene-specific primers) and PCR DNA polymerase and PCR components). Compared to one-step RT-qPCR, the disadvantages of two-step RT-qPCR include several steps for extended workflow, additional handling and processing of the sample, and increase the risk of contamination and variation results.

two-step-RT-PCR


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