Reactivos e instrumentos para inmunología, biología celular y biología molecular.
 

TransGen Biotech

TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. 

Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies. 
 
 
> TransGen Biotech > Productos para la investigación > Herramientas de biología molecular > Secuenciación de próxima generación (NGS) > NGS Library quantification products > Library quantifcation kits for Illumina® > Library quantifcation kits for Illumina® with standards
Library quantifcation kits for Illumina® with standards

Library quantifcation kits for Illumina® with standards


Accurate quantitation of the number of molecules in a library is the most critical step in the preparation steps for next-generation sequencing (NGS) to obtain high-quality reading data. Indeed, in order to obtain reliable and quality reading data, it is necessary to use a sufficient quantity of DNA, and in particular for Illumina® sequencing. The use of insufficient DNA will result in low sequencing efficiency and DNA overabundance will increase cluster density and result in poor quality data.

Quantitative PCR is considered to be the most accurate and efficient method of quantification of NGS library.

The library quantitation kits for Illumina® sequencing contain all the components necessary for the qPCR quantification of libraries:
  • Mix qPCR containing DNA polymerase, dNTPs and buffer
  • Primers to amplify only the molecules that contain the two sequences of adapters
  • The DNA standards (S1 to S6)
  • A ROX solution to add depending on the device used. If the ROX solution is separate from the mix, it is a universal kit that can be used with all instruments.

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