Cross adsorbed Anti-Rat secondary antibodies
Cross-adsorbed anti-rat antibodies are secondary antibodies that have been purified to remove antibodies that recognize shared epitopes on other proteins, in order to minimize cross-reactivity. Cross-adsorption or pre-adsorption is a purification step that increases the specificity of the antibody, resulting in higher sensitivity and less background staining. The cross-adsorption process involves binding the antibodies to target proteins that have been immobilized on solid-phase gels, and then further purifying the antibodies on similar gels to remove those that recognize shared epitopes on other proteins.
There are different types of cross-adsorbed anti-rat antibodies available, including goat anti-rat IgG (H+L) highly cross-adsorbed, goat anti-rat IgG (H+L) cross-adsorbed, and donkey anti-rat IgG (H+L) highly cross-adsorbed. These antibodies are suitable for various applications, such as western blotting, immunohistochemistry, immunocytochemistry, flow cytometry, and ELISA. The choice of antibody depends on the specific application and the level of cross-reactivity that needs to be minimized. Using antibodies that are extensively cross-adsorbed may result in diminished signal due to a reduced number of paratopes against the intended target.
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- Unconjugated 62
- FITC 45
- Biotin 41
- HRP 41
- AP 39
- PE 21
- Red 12
- Rhodamine 12
- RPE 8
- TRITC 8
- Texas Red® 8
- Colloidal Gold 7
- DyLight 488 7
- DyLight 550 7
- DyLight 594 7
- DyLight 650 7
- DyLight® 550 6
- DyLight® 594 6
- AMCA 5
- Cy3 5
- Cy5 5
- DyLight® 488 5
- DyLight® 650 5
- APC 4
- Cy2 4
- DyLight 680 3
- DyLight 755 3
- DyLight 800 3
- AbFluor™ 488 2
- Alexa Fluor 488 2
- Alexa Fluor 555 2
- Alexa Fluor 647 2
- Atto 425 2
- Atto 488 2
- Atto 532 2
- Atto 550 2
- Atto 594 2
- Atto 647N 2
- Atto 655 2
- BGAL 2
- Cy5.5 2
- SPRD 2
- AF647 1
- Cy3.5 1
- DyLight® 405 1
- LE/AF 1
- azide free 1
- rat 422
- mouse 6
- all species 1
- Secondary antibody
- goat 223
- donkey 52
- rabbit 52
- horse 11
- mouse 3
- ELISA 146
- IHC 122
- FC 107
- WB 74
- IF 70
- FLISA 64
- Microscopy 41
- ICC 24
- ChIP 1
- Mass spectrometry 1
- array 1
- Polyclonal 393
- polyclonal 6