The glutamate dehydrogenase activity assay is a vital tool for measuring the activity of the enzyme that catalyzes the reversible conversion of glutamate to α-ketoglutarate and ammonia. This assay is significant in various fields, including clinical diagnostics, metabolic research, and food safety.

Glutamate dehydrogenase is a mitochondrial enzyme involved in amino acid metabolism and plays a crucial role in the tricarboxylic acid cycle. It is allosterically regulated by various metabolites, including ADP, which activates it, and GTP and ATP, which inhibit its activity. Glutamate dehydrogenase is essential for maintaining nitrogen balance and energy production in cells.

Applications of Glutamate dehydrogenase Activity Assays

• Clinical Diagnostics: Elevated glutamate dehydrogenase levels can indicate metabolic disorders or tissue damage. For instance, abnormal glutamate dehydrogenase activity may be associated with conditions like congenital hyperinsulinism.
• Metabolic Research: Researchers use glutamate dehydrogenase assays to study metabolic pathways involving amino acids and their regulation under different physiological conditions.
• Food Safety and Analysis: Glutamate dehydrogenase assays are employed to assess food quality by determining glutamate levels in various food products.