NAD/NADH Assay Kits are critical tools for quantifying cellular levels of nicotinamide adenine dinucleotide (NAD⁺) and its reduced form (NADH), which are central to redox reactions, energy metabolism, and cellular signaling. These kits enable researchers to measure total NAD⁺/NADH, individual concentrations, and their ratios across diverse biological samples.

Types of NAD/NADH Assay Kits

Colorimetric Kits : Enzymatic cycling reactions produce a colorimetric signal proportional to NAD⁺/NADH levels, measured via absorbance.

Fluorometric Kits : NADH-specific fluorogenic probes emit fluorescence.

Key Features and Comparisons

• Metabolic Studies: Measuring NAD⁺/NADH ratios in cancer cells (e.g., SW480 colon cancer cells) to assess redox states.
• Drug Screening: Evaluating inhibitors like FK866, which targets NAD biosynthesis enzyme NAMPT.
• Tissue Analysis: Quantifying NADH/NAD⁺ ratios in muscle tissue under dietary interventions.

NAD/NADH assay kits offer versatile, high-precision tools for metabolic research, with options tailored for specific sample types and throughput needs. Their ability to resolve subtle changes in redox states makes them indispensable for studying cellular health, disease mechanisms, and therapeutic interventions.