Sequencing of mRNA by NGS - Library preparation - Modules

Sequencing of mRNA by NGS - Library preparation - Modules


Total RNA is composed of large amounts of unwanted transcripts, such as ribosomal RNA (rRNA), which accounts for 80-98% of a total RNA sample, and globin mRNA, which accounts for 35-80% of the mRNA in blood samples. Ribosomal RNA is a type of non-coding RNA that is the main component of ribosomes, essential to all cells. Ribosomal RNA is transcribed from ribosomal DNA (rDNA) and then bound to ribosomal proteins to form small and large ribosomal subunits. rRNA is the physical and mechanical component of the ribosome that forces the transfer RNA (tRNA) and messenger RNA (mRNA) to process and translate them into proteins.
Depletion of ribosomal RNA (rRNA) before RNA sequencing (Seq-RNA) is therefore essential and allows us to focus on the analysis of the portions of interest in the transcriptome. The use of oligo dT primer to capture the polyadenylated 3′ tail of transcripts and isolate the mRNA is common in many RNA sequencing preparations; however, this method does not allow processing of degraded samples where most of the RNA is separated from the 3′ tail, nor does it allow isolation of non-polyadenylated transcripts such as ncRNAs. Ribosomal removal methods address these problems by directly depleting rRNA while leaving other transcripts intact.

Resultados de su búsqueda : 21 Producto encontrado

Refine su búsqueda :

RUOCE / IVD
  • Unconjugated 3
  • Beads 1
  • Microspheres/Particles 10
  • kit 7
  • Buffers and reagents 4
  • PCR 3
APLICAR FILTROS
REINICIE


Referencia
Descripción
Cond.
Precio Sin IVA
NB-54-0061-02
 40ml 
NB-54-0061-03
 450ml 
NB-54-0061-01
 5ml 
N412-02
 40ml 
N412-01
 5ml 
N403-02
 96rxns 
N403-01
 24rxns 
EC501-02
 5ml 
EC501-01
 1ml 
EC501-03
 60ml 
N412-03
 450ml 
abx298002-5ml
 5ml 
abx298002-60ml
 60ml 
abx298002-1ml
 1ml 
D510
 50Preparations 
NB-54-0338-02
 96rxns