HRP Chromogen for IHC - DAB
DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).
Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling
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RUOCE / IVD
- Unconjugated 1116
- HRP 107
- FITC 69
- Biotin 68
- PE 55
- APC 50
- MaxLight 490 31
- MaxLight 550 31
- MaxLight 650 31
- MaxLight 405 18
- MaxLight 750 18
- Cy3 15
- Cy5 9
- Cy5.5 9
- Cy7 9
- Alexa Fluor 350 8
- Alexa Fluor 488 8
- Alexa Fluor 555 8
- Alexa Fluor 750 8
- PerCp 2
- RBITC 2
- APC-CY5.5 1
- APC-Cy7 1
- APC/Cy5.5 1
- APC/Cy7 1
- Alexa Fluor 405 1
- Alexa Fluor 594 1
- Alexa Fluor 647 1
- Alexa Fluor 680 1
- PE-Cy5 1
- PE-Cy5.5 1
- PE-Cy7 1
- PE/Cy5 1
- PE/Cy5.5 1
- PE/Cy7 1
- PerCP-Cy5.5 1
- PerCP/Cy5.5 1
- human 1090
- mouse 361
- rat 316
- e. coli 53
- cow 50
- dog 47
- pig 47
- cat 26
- rabbit 24
- horse 23
- monkey 19
- sheep 18
- camel 16
- hamster 16
- guinea pig 7
- chicken 6
- plant 5
- donkey 4
- duck 4
- fish 4
- goose 4
- pigeon 4
- monosan dab high contrast 2
- xenopus 2
- zebrafish 2
- 1 1
- monosan plus (hrp) broad spectrum (dab)kit 1
- rabbit 633
- mouse 438
- human 353
- e. coli 146
- yeast 122
- 48
- rat 40
- goat 36
- horse 12
- dog 10
- hamster 10
- sheep 10
- virus 9
- cow 8
- monkey 8
- pig 8
- bird 5
- chicken 5
- cat 1
- donkey 1
- guinea pig 1
- Primary antibody 1246
- Biochemicals 1190
- Protein/peptide 1000
- ELISA/ELISPOT 367
- Inhibitor/Antagonist/Agonist 324
- Buffers and reagents 159
- CRISPR/Cas9 149
- RNAi 102
- Probe 62
- Destination vector 50
- kit 40
- Other products 36
- Secondary antibody 12
- Cell line 6
- cDNA Clone 4
- Lysate 2
- Paraffin tissue section 1
- Primer 1
- WB 762
- ELISA 754
- IHC 471
- FLISA 121
- ICC 110
- IF 104
- IP 101
- FC 87
- Enzyme-linked immunosorbent assay for Antigen Detection. 10
- 0.078 ng/mL 3
- Chemiluminescence 3
- 0.1 ng/mL 2
- 0.116 ng/ml 1
- ISH 1
- PCR 1
- Polyclonal 661
- 2G6 31
- 4G5 31
- 1F8 30
- 1G1 30
- 1G5 30
- 10i46 25
- 13H261 21
- 3H1166 21
- Monoclonal 21
- M-A261 12
- HLy9.1.25 8
- 1626CT702.263.21 5
- 1A10A10 5
- 10.1F8 4
- 12H6.D3 4
- 13.1G11 4
- 13.2G8 4
- 13.6D9 4
- 13.7E2 4
- 13.7H4 4
- 13.8E5 4
- 13B4.E10 4
- 14.2H9 4
- 14F10.H9 4
- 1A6 4
- 26.7C4 4
- 2E4 4
- 3G8 4
- 4A8 4
- 4B9 4
- 5A11 4
- 5A2 4
- 6.1H3.D9.C6 4
- 6.1H8.F9.G6 4
- 6.2D2.E1.D11 4
- 6.7A2.A4.D3 4
- 6.8A2.B8.A11 4
- 6.8E7.A7.A1 4
- 8C7 4
- 8G1 4
- 8H12 4
- 8H6 4
- 9.21C1.G1.F1.A5 4
- 9.22E1.F5.H10 4
- 9.24E10.F3.H12 4
- IgG.2A5 4
- IgG.2E9 4
- Not Applicable 4
- RB21227 4
- 6D23 3
- SAA1697 3
- 12K274 2
- 17D17 2
- 17L18 2
- 202.36 2
- 2213 2
- 2M2 2
- 3F9-2C2 2
- 5B20 2
- 7H111 2
- 8C10 2
- B2M-01 2
- B2M-02 2
- 11C257 1
- 12K99 1
- 134-2C2 1
- 13B822 1
- 5K78 1
- 5K78 (134-2C2) 1
- 6C1 1
- 6C5 1
- 9H177 1
- ADAB-8 1
- DAB-6 1
- Recombinant 1
Referencia
Descripción
Cond.
Precio Sin IVA
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