Tryptone agar is a non-selective medium used for the determination of motility and fermentation capacity of aerobic and anaerobic bacteria. It is recommended for Clostridia, Bacillus species, Micrococci, enteric bacilli and other non-proliferating organisms. This medium also serves as a base which with supplements can be used for a wide variety of demanding microorganisms. Tryptone is a pancreatic digestion product of casein and as such is composed of a mixture of amino acids, including essential amino acids, and larger peptides. Tryptone agar base is also an excellent medium for the maintenance of aerobic and anaerobic cultures. Fermentation reactions can be determined by adding the desired carbohydrates. Acid production during fermentation is detected by the phenol red indicator by changing the color of the medium from red to yellow.