AP Chromogen for IHC - NBT/BCIP
Combination of NBT (nitro blue tetrazolium chloride) and BCIP (5-brom-4-chloro-3'-indolyphosphate p-toluidine salt) makes it possible to obtain an intense black violet insoluble precipitate when it reacts with alkaline phosphatase, an enzyme widely used for immunoassays such as IHC, WB or ELISA.
These substrates thus permit detect alkaline phosphatase activity on a solid surface, such as nitrocellulose membranes or tissue sections.
The precipitate produced is partially insoluble in alcohol. This combination of substrates is more sensitive than other substrates of alkaline phosphatase such as AP-Red or AP-Blue.
Name
NBT/BCIP
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
Alkaline phosphatase
Reaction type
Precipitating Reaction
Precipitate color
Red
Microscopy
Brightfield, Fluorescence, Spectral Imaging
Mounting
Aqueous
Other properties
Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
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RUOCE / IVD
- Unconjugated 39
- Buffers and reagents 42
- kit 12
- Biochemicals 7
- ELISA/ELISPOT 1
- IHC 16
- WB 14
- Assay 4
- ISH 3
- CISH-P 1
- IF 1
Referencia
Descripción
Cond.
Precio Sin IVA
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