Reactivos e instrumentos para inmunología, biología celular y biología molecular.

LymphoTranscript for the detection of over 50 fusion transcripts

The genetic of peripheral T-cell lymphoma (PTCL) is intricate, involving numerous recurrent fusion transcripts identified in recent years through high-throughput sequencing. Despite these advancements, an affordable and rapid method for their simultaneous detection in clinical samples remains unavailable.

The LymphoTranscript enables the detection of fusion transcripts associated with peripheral T-cell lymphomas in a single test. It is a cost-effective assay.

The assay utilizes a ligation-dependent RT-PCR (LD-RT-PCR) method, a semi-quantitative technique that enables the simultaneous analysis of chromosomal translocations and somatic mutations. This is achieved through the use of oligonucleotide probe pairs designed to specifically target each marker.

A simple and rapid protocol

The In vitro test involves four steps, with a total duration of about half a day, including 1 to 1.5 hours of hands-on time.

The NGS sequencing requires only 100,000 reads per sample.

Additionally, the LymphoTranscript test can be sequenced alongside other libraries, with barcodes provided in the kit.

Post sequencing analysis

After sequencing, the RT-MIS online analysis platform facilitates the automated analysis of FASTQ files. It then generates a list of detected fusions in the biological sample, along with the corresponding quantitative data. RT-MIS also provides the relevant bibliography for these fusions.

Thus, RT-MIS offers a simple, fast, and secure solution for users.

Characteristics of LymphoTranscript

Application domain	Fusion transcript detection
Handling duration	≃4h before sequencing
Type of samples	Fresh, frozen or fixed and paraffin-embedded tissue biopsies
Input quantity	Between 50 and 500ng in a volume of 2μL
Contents of the reagent kit	Probes targeting fusion transcripts, somatic mutations and HTLV1 virus, barcodes, sequencing primers

Product details

Cat. No.	Description	Cond.
GEP-LT08	LymphoTranscript Kit	8reactions
GEP-LT16	LymphoTranscript Kit	16reactions
GEP-LT24	LymphoTranscript Kit	24reactions