Custom Peptide Synthesis

Custom peptide production service offers the synthesis and purification of peptides. This peptide synthesis can be accompanied by modifications at the N or C terminal positions.

Peptide synthesis typically involves coupling the carboxyl group of an incoming amino acid to the N-terminus of the growing peptide chain, following a C-to-N direction. This approach contrasts with protein biosynthesis, where amino acids are added in the N-to-C direction by linking the N-terminus of the incoming amino acid to the C-terminus of the growing protein chain. In vitro peptide synthesis, due to its complexity, proceeds in a precise, stepwise, and cyclic manner. Despite variations among commonly used peptide synthesis methods, they all share this sequential approach, adding amino acids one at a time to the peptide chain.

The peptide synthesis service adapts to your request:

• Simple linear peptides
• Complex peptides; cyclic,
• Long chain peptides (up to 112 amino acids)
• Modified amino acid containing peptides
• Various grades of purity: crude, desalted, >75%、>85%、>90%、>95%、>98%.

 

 

Choose the purity of your peptide according to your needs:

Crude (>50 >70%)

 

• High-throughput screening of lead compounds.
• Mutation screening
• Sequence optimization

 

Immuno-grade purity (>75%)

 

• ELISA testing
• Peptide arrays
• Production, purification and testing of antibodies used in immunoassays

 

Biochemistry grade ( >85%)

 

• For in-vitro bioassays and semi-quantitative applications (non-quantitative) such as studies in enzymology, epitope mapping, biological activity, enzyme-substrate interactions, or phosphorylation, as well as for peptide blocking studies for Western Blot and cell attachment.
• Antigens for polyclonal antibody production
• Coupling to chromatography resins for affinity purifications

 

High purity: >90% to >98%

 

• Production of monoclonal antibodies
• In vitro quantitative studies such as receptor-ligand interaction studies, enzyme-substrate and other biological activity studies, phosphorylation and proteolysis studies
• Quantitative blocking and competitive inhibition assays, electrophoresis markers and chromatography standards
• Quantitative bioassays (ELISA and RIA)
• In vivo studies

 

Extremely high purity: >98%

 

• In vivo studies, clinical trials, drug studies that use peptides as pharmaceuticals and cosmetic peptides for cosmeceuticals industry
• Structure-activity relationship studies (enzyme-substrate studies, receptor-ligand interaction studies…)
• Protein structure studies by nuclear magnetic resonance or protein crystallography.

 

The average time to synthesize a peptide is 2 to 3 weeks.

The peptide is analyzed by mass spectrometry, HPLC and is delivered with a synthesis report.
 

Peptides may have the following modifications (non-exhaustive list) :

 

• In N-terminal
• In C terminal
• Non-usual amino acids
• Conjugation