TransGen Biotech
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TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
Website : www.transgenbiotech.com
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![One-Step RT-qPCR mixes - SYBR Green](https://www.clinisciences.com/es/upload/thumbs/bandeau4-jnutf3-e3ccf8.png)
One-Step RT-qPCR mixes - SYBR Green
As its name implies, single-step RT-PCR combines the synthesis of complementary DNA (cDNA) of the first strand (reverse transcript, RT) and subsequent PCR in a single reaction tube. This reaction configuration simplifies the work, reduces variations and minimizes possible contamination. One-step RT-PCR makes it easier to process a large number of samples, allowing for high-throughput applications. However, one-step RT-PCR uses specific gene primers for amplification, limiting the analysis to a few genes per RNA sample. Since the reaction is a compromise between reverse transcription and amplification conditions, one-step RT-PCR may be less sensitive and less effective in some cases. However, the use of a gene-specific primer in RT-PCR can help maximize the yield of the target cDNA and minimize amplification.
![One-step-RT-PCR](/upload/one-step-rt-pcr-cayvxr.jpg)
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