Abbexa

 
Abbexa es una empresa biotecnológica especializada en reactivos de investigación fiables y de alta calidad para la investigación científica. Aportando una fuente integrada de anticuerpos, proteínas, kits ELISA y otros reactivos biológicos, la Compañía ofrece soporte técnico experto, asegurando una excelente calidad a través de una extensa validación.

 

 
Página web : www.abbexa.com
 
IHC buffers - Antigen retrieval

IHC buffers - Antigen retrieval


Most formalin-fixed tissues require an antigen retrieval step before immunohistochemical staining. Although fixation is essential for the preservation of tissue morphology, this process can also have a negative impact on IHC/ICC detection. Fixation can alter protein biochemistry such that the epitope of interest is masked and can no longer bind to the primary antibody. Methylene bridges formed during fixation cross-link proteins and mask antigenic sites. Antigen retrieval methods break these methylene bridges and expose antigenic sites, allowing antibodies to bind. The two methods for antigen retrieval are heat induced epitope retrieval (HIER) and enzymatic retrieval.

We offer a range of HIER Retrieval Buffers with different pH and packaging (ready-to-use or concentrated) to find the one that best suits your protocol.


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