Agarose Bead Technologies (ABT)
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Agarose Bead Technologies (ABT) se dedica a la investigación y el desarrollo de productos biotecnológicos derivados de la agarosa. La empresa ofrece una amplia gama de microesferas de agarosa no activadas para la exclusión por tamaño, así como microesferas de agarosa activadas para la cromatografía de afinidad y de intercambio iónico (purificaciones de proteínas y anticuerpos), y resinas preactivadas para el acoplamiento de ligandos de afinidad.
ABT es una empresa certificada ISO 9001:2015 y se basa en un sólido programa de control de calidad.
Página web: www.abtbeads.es
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Protein A Affinity resins
Protein A is a cell wall protein from Staphylococcus aureus with a molecular weight between 35-50 kDa and shows high specificity for the Fc region of immunoglobulin molecules of many mammalian species. It is encoded by the spa gene, and its regulation is carried out at the level of the DNA topology, as a function of cellular osmolarity, by a two-component system called ArlS-ArlR. The quality of the resin (Protein A Agarose or equivalent) and stability of the immobilisation is important to avoid leakage of Protein A during the elution procedure.
Protein A affinity chromatography is a rapid one-step purification, which removes most nonIgG contaminants and can achieve purities close to homogeneity. It is particularly useful for purifications of tissue culture supernatant, where 10-100 fold concentrations can be achieved.
Specifications:
Protein A affinity chromatography is a rapid one-step purification, which removes most nonIgG contaminants and can achieve purities close to homogeneity. It is particularly useful for purifications of tissue culture supernatant, where 10-100 fold concentrations can be achieved.
Specifications:
- Resin: Protein A Agarose F
- Base Matrix: Agarose
- Protein A capacity (hIgG): 30 mg (per 1 ml resin)
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