SERVA Electrophoresis
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50 years of experience in the supply of quality reagents at SERVA provides expertise in manufacture, development and distribution of products worldwide. This know-how is the platform for outstanding customer service - all you need to succeed. Our mission is to bring innovative products to the life sciences market, meeting our customers needs. Technical competence and total quality management are the basis for continuous improvement. We pursue the highest standards in product quality, workplace safety and responsibility for the environment we live in. We dedicate our capacity and integrity to guarantee consistent product performance and continuity of supply. We are certified for ISO 9001:2000.
Website : www.serva.de |
![Protein A Affinity resins](https://www.clinisciences.com/es/upload/thumbs/bandeau3-svyndgvx-SUEpcHXb-f3e279.png)
Protein A Affinity resins
Protein A is a cell wall protein from Staphylococcus aureus with a molecular weight between 35-50 kDa and shows high specificity for the Fc region of immunoglobulin molecules of many mammalian species. It is encoded by the spa gene, and its regulation is carried out at the level of the DNA topology, as a function of cellular osmolarity, by a two-component system called ArlS-ArlR. The quality of the resin (Protein A Agarose or equivalent) and stability of the immobilisation is important to avoid leakage of Protein A during the elution procedure.
Protein A affinity chromatography is a rapid one-step purification, which removes most nonIgG contaminants and can achieve purities close to homogeneity. It is particularly useful for purifications of tissue culture supernatant, where 10-100 fold concentrations can be achieved.
![Ni-NTA-coordination](/upload/proteina-agarose-fufv3k-ssNAbh5T.png)
Specifications:
Protein A affinity chromatography is a rapid one-step purification, which removes most nonIgG contaminants and can achieve purities close to homogeneity. It is particularly useful for purifications of tissue culture supernatant, where 10-100 fold concentrations can be achieved.
![Ni-NTA-coordination](/upload/proteina-agarose-fufv3k-ssNAbh5T.png)
Specifications:
- Resin: Protein A Agarose F
- Base Matrix: Agarose
- Protein A capacity (hIgG): 30 mg (per 1 ml resin)
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