ReproCELL
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Integrated Tools for Translational Research 3D Cell Culture Alvetex's highly porous polystyrene scaffold enables your cells to maintain their in vivo morphology, behavior and responsiveness. Stemgent Innovative reagents for stem cell research and cellular reprogramming Stemgent RNA reprogramming systems RNA reprogramming systems for fibroblasts, blood and urine ReproCELL Neurons ReproNeuro : the world's first human neurons derived from iPS cells ReproCELL cardiomyocytes The world's first commercial human iPSC-derived cardiomyocytes ReproCELL Hepatocytes The world's first commercial human iPSC-derived hepatocytes. Robust CYP activity and other typical characteristics of human hepatic cells. ReproCELL Stem Cell Media A range of media and reagents specifically developed to provide superior results in human ES and iPS culture website : www.reprocell.com |
![Dulbecco’s Modified Eagle Medium (DMEM)](https://www.clinisciences.com/es/upload/thumbs/bandeau3-dn3mkzpw-KbXc92sS-553b68.png)
Dulbecco’s Modified Eagle Medium (DMEM)
Dulbecco's Modified Eagle Medium (DMEM) stands as a cornerstone in cell culture research, facilitating the proliferation of diverse mammalian cell lines. Initially designed with modest glucose levels (1 g/L) and the inclusion of sodium pyruvate, DMEM has evolved to offer a spectrum of formulations catering to varied experimental needs.
DMEM's superiority over Eagle's Minimal Essential Medium lies in its augmented provision of essential nutrients and growth factors, with concentrations of amino acids and vitamins quadrupling that of the latter. Its formulation incorporating a sodium bicarbonate buffer system ensures stable physiological pH within the optimal CO2 environment of 5–10%.
However, the versatility of DMEM necessitates careful consideration during selection. Factors such as the presence of L-glutamine or its superior variant GlutaMAX, the inclusion of phenol red as an indicator, and the choice between liquid and powder formats warrant attention. Additionally, decisions regarding serum supplementation, sodium pyruvate, glucose levels, and the incorporation of HEPES buffer should align with the specific requirements of the experimental setup.
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