Anti-GJA1 antibody (332-381 aa)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	GJA1
Gene ID:	2697
Uniprot ID:	CXA1_HUMAN
Immunogen Region:	332-381 aa
Specificity:	Connexin 43 Polyclonal Antibody detects endogenous levels of Connexin 43 protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human Connexin 43 at the amino acid range 332-381

Post Translational Modifications	Phosphorylated at Ser-368 by PRKCG.phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity. Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation. Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2. S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication. Acetylated in the developing cortex.leading to delocalization from the cell membrane.
Function	Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles.
Protein Name	Gap Junction Alpha-1 Protein Connexin-43 Cx43 Gap Junction 43 Kda Heart Protein
Database Links	Reactome: R-HSA-190704 Reactome: R-HSA-190827 Reactome: R-HSA-190840 Reactome: R-HSA-190861 Reactome: R-HSA-190873 Reactome: R-HSA-191650 Reactome: R-HSA-196025 Reactome: R-HSA-9013406 Reactome: R-HSA-9013409 Reactome: R-HSA-9705677
Cellular Localisation	Cell Membrane Multi-Pass Membrane Protein Cell Junction Gap Junction Endoplasmic Reticulum Localizes At The Intercalated Disk (Icd) In Cardiomyocytes And The Proper Localization At Icd Is Dependent On Tmem65
Alternative Antibody Names	Anti-Gap Junction Alpha-1 Protein antibody Anti-Connexin-43 antibody Anti-Cx43 antibody Anti-Gap Junction 43 Kda Heart Protein antibody Anti-GJA1 antibody Anti-GJAL antibody

Information sourced from Uniprot.org

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