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Reactivos e instrumentos para inmunología, biología celular y biología molecular.

Anti-WNT1 antibody (231-370)

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Referencia NB-22-33237-200

embalaje : 200uL(2x100uL)

Contact local distributor :

Teléfono : +1 850 650 7790

Marca : Neo Biotech

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Contact local distributor :

Teléfono : +1 850 650 7790

Immunofluorescence analysis of PC-12 cells using WNT1 Rabbit polyclonal antibody at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using WNT1 Rabbit polyclonal antibody at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 cells using WNT1 Rabbit polyclonal antibody at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of various lysates using WNT1 Rabbit polyclonal antibody at 1:8000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 60s.

General Info

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-WNT1 (231-370) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:1000-1:4000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	WNT1
Gene ID:	7471
Uniprot ID:	WNT1_HUMAN
Immunogen Region:	231-370
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 231-370 of human WNT1 (NP_005421.1).
Immunogen Sequence:	TCWMRLPTLRAVGDVLRDRF DGASRVLYGNRGSNRASRAE LLRLEPEDPAHKPPSPHDLV YFEKSPNFCTYSGRLGTAGT AGRACNSSSPALDGCELLCC GRGHRTRTQRVTERCNCTFH WCCHVSCRNCTHTRVLHECL

Description

Post Translational Modifications	Palmitoleoylation is required for efficient binding to frizzled receptors. Palmitoleoylation is necessary for proper trafficking to cell surface (Probable). Depalmitoleoylated by NOTUM, leading to inhibit Wnt signaling pathway.
Function	Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Plays an essential role in the development of the embryonic brain and central nervous system (CNS). Has a role in osteoblast function, bone development and bone homeostasis.
Protein Name	Proto-Oncogene Wnt-1 Proto-Oncogene Int-1 Homolog
Database Links	Reactome: R-HSA-201681 Reactome: R-HSA-3238698 Reactome: R-HSA-373080 Reactome: R-HSA-381340 Reactome: R-HSA-4086400 Reactome: R-HSA-4641262
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative Antibody Names	Anti-Proto-Oncogene Wnt-1 antibody Anti-Proto-Oncogene Int-1 Homolog antibody Anti-WNT1 antibody Anti-INT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance