Biological Activity

Blebbistatin is a cell-permeable inhibitor for non muscle myosin II ATPase with IC50 of ~2 μM, does not inhibit myosin light chain kinase, inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly.

*The compound is unstable in solutions, freshly prepared is recommended

Product Citations

• 

  Lab Chip. 2023 Jul 7.

  A microfluidic platform integrating dynamic cell culture and dielectrophoretic manipulation for in situ assessment of endothelial cell mechanics
  (-)-Blebbistatin purchased from AbMole

Chemical Information

Molecular Weight	292.33
Formula	C18H16N2O2
CAS Number	856925-71-8
Solubility (25°C)	DMSO 10 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Wener Li, et al. Blebbistatin protects iPSC-CMs from hypercontraction and facilitates automated patch-clamp based electrophysiological study

[2] Song Gao, et al. Blebbistatin Inhibits Neomycin-Induced Apoptosis in Hair Cell-Like HEI-OC-1 Cells and in Cochlear Hair Cells

[3] Yuanyuan Zhang, et al. An inhibitor of myosin II, blebbistatin, suppresses development of arterial thrombosis

[4] Anna Rauscher, et al. Targeting Myosin by Blebbistatin Derivatives: Optimization and Pharmacological Potential

[5] Bart I Roman, et al. Medicinal Chemistry and Use of Myosin II Inhibitor ( S)-Blebbistatin and Its Derivatives