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> CD11b antibody [ICRF44] (PE)

CD11b antibody [ICRF44] (PE)

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Referencia GTX00481-08-100test

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Marca : GeneTex

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Anti-CD11b antibody [ICRF44] (PE) used in Flow cytometry (FACS). GTX00481-08

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 ICRF44

Isotype

 IgG1

Application

 FACS

Reactivity

 Human, Pig, Baboon, Chimpanzee, Cynomolgus monkey, Rhesus Monkey
Package
100 test
Datasheet File
View product citations for antibody GTX00481-08 on CiteAb
PRODUCT

Summary

The ICRF44 antibody reacts with human CD11b, also known as integrin αalpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin αreceptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrin αs are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin αreceptors consist of distinct combinations of integrin αchains which are differentially expressed. integrin αalpha M (CD11b) assembles with integrin αbeta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1, ICAM-2, ICAM-3 and ICAM-4 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 1 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rheumatoid synovial cells and human monocytes.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin (PE) Wavelength

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha M , CD11B , CR3A , MAC-1 , MAC1A , MO1A , SLEB6

Cellular Localization

Cell membrane

Background

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

DATA IMAGES

Anti-CD11b antibody [ICRF44] (PE) used in Flow cytometry (FACS). GTX00481-08

GTX00481-08 FACS Image

FACS analysis of human peripheral blood monocytes using GTX00481-08 CD11b antibody [ICRF44] (PE).
Solid line : Primary antibody
Dashed line : PE mouse IgG1 isotype control
Antibody amount : 1 μg

Clone Reference

2017; Dyslipidemic Diet-Induced Monocyte "Priming" and Dysfunction in Non-Human Primates Is Triggered by Elevated Plasma Cholesterol and Accompanied by Altered Histone Acetylation
2016; Vitamin D machinery and metabolism in porcine adipose-derived mesenchymal stem cells
2015; Affinity maturation of a novel antagonistic human monoclonal antibody with a long VH CDR3 targeting the Class A GPCR formyl-peptide receptor 1
2013; Glioma grade is associated with the accumulation and activity of cells bearing M2 monocyte markers
2011; Endogenous PMN sialidase activity exposes activation epitope on CD11b/CD18 which enhances its binding interaction with ICAM-1
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REVIEW

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Clone Reference
2017; Dyslipidemic Diet-Induced Monocyte "Priming" and Dysfunction in Non-Human Primates Is Triggered by Elevated Plasma Cholesterol and Accompanied by Altered Histone Acetylation
2016; Vitamin D machinery and metabolism in porcine adipose-derived mesenchymal stem cells
2015; Affinity maturation of a novel antagonistic human monoclonal antibody with a long VH CDR3 targeting the Class A GPCR formyl-peptide receptor 1
2013; Glioma grade is associated with the accumulation and activity of cells bearing M2 monocyte markers
2011; Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation
2011; Endogenous PMN sialidase activity exposes activation epitope on CD11b/CD18 which enhances its binding interaction with ICAM-1
2010; Attenuation of innate immunity by cytomegalovirus IL-10 establishes a long-term deficit of adaptive antiviral immunity
2009; Tolerogenic dendritic cells actively inhibit T cells through heme oxygenase-1 in rodents and in nonhuman primates
2004; Integrins are not involved in the process of human sperm-oolemmal fusion
2003; Interaction of proteinase 3 with CD11b/CD18 (beta2 integrin) on the cell membrane of human neutrophils
2001; Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB
1998; A combination of osteoclast differentiation factor and macrophage-colony stimulating factor is sufficient for both human and mouse osteoclast formation in vitro
SDS
PBS.pdf
Sodium Azide.pdf