CDCA8 (1-280) Mouse Monoclonal Antibody [Clone ID: 1D11]
Specifications
Product Data | |
Clone Name | 1D11 |
Applications | IF, IP, WB |
Recommended Dilution | Western blot: 1 µg/ml for chemiluminescence detection system. Immunoprecipitation: 1 µg/50 µl of cell extract from 5 x 106 cells. Immunoflourescence: 5 µg/ml. For details see protocols below. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Recombinant full-length human Borealin (1-280 aa) |
Specificity | This antibody reacts with Borealin (35 kDa). |
Formulation | PBS containing 50% glycerol, pH 7.2. No preservative is contained. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store (in aliqouts) at -20 °C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Gene Name | cell division cycle associated 8 |
Database Link | |
Background | Borealin is a subunit of the chromosomal passenger complex (CPC), which is necessary for chromosome segregation and cytokinesis. While CPC shows a dynamic localization pattern during mitosis, Borealin and survivin, another subunit of CPC is essential to localize CPC at centromare from prophase to anaphase. It is thought that Borealin and survivin recruit aurora B, a kinase subunit of CPC, to inner centromare to allow phosphorylating appropriate substrates. |
Synonyms | Dasra-B, hDasra-B, PESCRG3 |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting Immunoprecipitation Immunocytochemistry |
Reference Data |
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