- More Files
Cellular senescence was measured with the Cellular Senescence Activity Assay Kit using a (A) NovoCyte Flow Cytometer (ACEA Biosciences) and (B) fluorescence microscope.
Cellular senescence was measured with the Cellular Senescence Activity Assay Kit using a (A) NovoCyte Flow Cytometer (ACEA Biosciences) and (B) fluorescence microscope.
(A) HL-60 cells were incubated with Camptothecin for 6 hours to induce senescence and stained with Xite beta-D-galactopyranoside for 30 mins at 37°C. The signal was acquired using FITC channel in ACEA NovoCyte flow cytometer.
(B) HeLa cells were incubated with Camptothecin for 6 hours to induce senescence and stained with Xite beta-D-galactopyranoside for 30 mins at 37°C. The signal was acquired using FITC filter set.
- Specifications
Product Description
Cellular Senescence Activity Assay Kit uses Xite beta-D-galactopyranoside, a fluorogenic beta-Gal substrate that readily enters into live cells, and gets cleaved by SA-beta-gal inside cells, generating strong green fluorescence. Unlike cell-impermeable X-Gal substrate, it has excellent cell permeability. The Cellular Senescence Activity Assay Kit enables users to detect the senescence with higher sensitivity with robust performance.
Suitable Sample
Cells.
Detection Method
Fluorimetric
Platform
Instrument: Flow cytometer
Excitation: 488 nm laser
Emission: 530/30 nm filter
Instrument specification(s): FITC channel
Instrument: Fluorescence microscope
Excitation: FITC filter set
Emission: FITC filter set
Recommended plate: Black wall/clear bottomRegulatory Status
For research use only (RUO)
Storage Instruction
Store the kit at -20°C and avoid from light.
Note
Cellular senescence was measured with the Cellular Senescence Activity Assay Kit using a (A) NovoCyte Flow Cytometer (ACEA Biosciences) and (B) fluorescence microscope.
- Applications
Functional Study
Flow Cytometry