Datura stramonium (DSA) (FITC)

This 86,000 molecular weight glycoprotein contains two chains of 40,000 and 46,000D joined by disulfide bonds. This lectin is free of a reported 32,000 molecular weight contaminant protein. The carbohydrate binding site recognizes (b-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0. DSL is mitogenic toward human peripheral lymphocytes and its potency has been reported to increase as the lectin aggregates to larger molecular weight species. Datura lectin also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (b-1,6) and (b-1,2) was reported to be the most potent inhibitor of agglutination. Carcinoembryonic antigen, capsular polysaccharides and many glycoproteins have binding sites for this lectin. Fluorescein labeled Datura Stramonium Lectin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Datura Stramonium Lectin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.||Applications: |Suitable for use in FLISA. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Inhibiting/Eluting Sugar: |Chitin Hydrolysate||Excitation:|495nm ||Emission:|515nm||F/P (molar):|2||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.