EasyPure® HiPure Plasmid MiniPrep Kit
Referencia EM111-01
embalaje : 50rxns
Marca : TransGen Biotech
Contact local distributor :
Teléfono : +1 850 650 7790
ManualEasyPure® HiPure Plasmid MiniPrep Kit provides an efficient way to isolate high yield (up to 40 μg) and high quality plasmid DNA from ≤20 ml (LB) of bacterial culture. Unique formulated lysis buffer and neutralization buffer permit complete bacterial cell lysis and neutralization.Endotoxin is removed by a simple incubation on column with a novel buffer. The purified plasmid DNA is suitable for a variety of molecular biology applications, including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection.
• Fast: the whole procedure can be performed in 20 minutes.
• Simple: endotoxin is removed on column.
• High yield: DNA yield up to 40 μg.
Storage
at room temperature (15-25°C) in a dry place for one year.
Shipping
at room temperature.
| Component | EM111-01 (50 rxns) |
| Resuspension Buffer (RB) | 15 ml |
| Lysis Buffer (LB) | 15 ml |
| Neutralization Buffer (NB) | 20 ml |
| ToxinOut Buffer (TB) | 15 ml |
| Wash Buffer (WB) | 10 ml |
| Elution Buffer (EB) | 5 ml |
| RNase A (10 mg/ml) | 150 μl |
| Mini-Plasmid Spin Columns with Collection Tubes | 50 each |
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