Griffonia simplicifolia (GSL I)

Griffonia simplicifolia lectin (GSL I) is affinity purified from a woody climbing shrub native to Africa. GSL I is composed of two types of subunits A and B that combine to form tetrameric structures, resulting in five isolectins. This lectin has affinity for terminal alpha-D-galactosyl residues and terminal N-acetyl-alpha-D-galactosaminyl residues. The A-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for alpha-N-acetylgalactosamine residues, while the B-rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues. This lectin shows specificity for blood group B greater than blood group A1. GSL I has been reported to bind several glycoproteins including laminin. Since some alpha-D-galactosyl residues are expressed in endothelial cells of mouse tissues, GSL I can present a highly specific surface of glycosylation pattern in these cells.||Source:|Bandeiraea||Blood Specificity:|B >> A1||Sugar Specificity:|alpha-Galactose, alpha-N-Acetylgalactosamine||Inhibitory Carbohydrate:|Galactose, N-Acetylgalactosamine||Divalent Ions:|Ca++, Mn++||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.