GSK2194069 [1332331-08-4]
Referencia HY-12325-5mg
embalaje : 5mg
Marca : MedChemExpress
GSK2194069 is a potent inhibitor of β-ketoyl reductase (KR) of fatty acid synthase (FASN), with an IC50 value of 7.7 nM. GSK2194069 shows specifically inhibitory effect on FAS expressing cancer cells, by acting potent efficacy on acetoacetyl-CoA, NADPH with IC50 or Ki values of 4.8 nM and 5.6 nM, respectively.
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GSK2194069 Estructura química
No. CAS : 1332331-08-4
This product is a controlled substance and not for sale in your territory.
Based on 3 publication(s) in Google Scholar
Descripciòn |
GSK2194069 is a potent inhibitor of β-ketoyl reductase (KR) of fatty acid synthase (FASN), with an IC50 value of 7.7 nM. GSK2194069 shows specifically inhibitory effect on FAS expressing cancer cells, by acting potent efficacy on acetoacetyl-CoA, NADPH with IC50 or Ki values of 4.8 nM and 5.6 nM, respectively[1][2][3]. |
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In Vitro |
GSK2194069 (100 nM; 24 h) inhibits fatty acid synthase (FAS) in cancer cell lines (KATO-III, MKN45, A549, SNU-1) without reducing FAS production protein level[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot Analysis[1]
Western Blot Analysis[2]
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Peso molecular |
428.48 |
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Fòrmula |
C25H24N4O3 |
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No. CAS |
1332331-08-4 |
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Appearance |
Solid |
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Color |
Light yellow to khaki |
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SMILES |
O=C(N1C[C@H](CC2=NNC(N2C3=CC=C(C4=CC=C(OC=C5)C5=C4)C=C3)=O)CC1)C6CC6 |
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Envío | Room temperature in continental US; may vary elsewhere. |
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Almacenamiento |
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Solvente y solubilidad |
In Vitro:
DMSO : ≥ 100 mg/mL (233.38 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
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%
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%
Tween-80
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%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
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If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureza y Documentación |
Purity: 99.84% |
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Referencias |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 2.3338 mL | 11.6692 mL | 23.3383 mL | 58.3458 mL |
5 mM | 0.4668 mL | 2.3338 mL | 4.6677 mL | 11.6692 mL | |
10 mM | 0.2334 mL | 1.1669 mL | 2.3338 mL | 5.8346 mL | |
15 mM | 0.1556 mL | 0.7779 mL | 1.5559 mL | 3.8897 mL | |
20 mM | 0.1167 mL | 0.5835 mL | 1.1669 mL | 2.9173 mL | |
25 mM | 0.0934 mL | 0.4668 mL | 0.9335 mL | 2.3338 mL | |
30 mM | 0.0778 mL | 0.3890 mL | 0.7779 mL | 1.9449 mL | |
40 mM | 0.0583 mL | 0.2917 mL | 0.5835 mL | 1.4586 mL | |
50 mM | 0.0467 mL | 0.2334 mL | 0.4668 mL | 1.1669 mL | |
60 mM | 0.0389 mL | 0.1945 mL | 0.3890 mL | 0.9724 mL | |
80 mM | 0.0292 mL | 0.1459 mL | 0.2917 mL | 0.7293 mL | |
100 mM | 0.0233 mL | 0.1167 mL | 0.2334 mL | 0.5835 mL |
GSK2194069 Related Classifications
- Metabolic Enzyme/Protease
- Fatty Acid Synthase (FASN)