Heat Shock Protein 70, Recombinant Human (Hsp70)

Referencia H1830-95T7-100ug

embalaje : 100ug

Marca : US Biological

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H1830-95T7 Heat Shock Protein 70, Recombinant Human (Hsp70)

Clone Type
Polyclonal
Grade
Affinity Purified
Applications
WB
Shipping Temp
Blue Ice
Storage Temp
-20°C

Hsp70 genes encode abundant heat-inducible 70kD hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded |intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and |protein transport.||Source: |Protein with ATPase activity, his-tagged, Recombinant Hsp70 cloned from a human cDNA library and expressed in E.coli.||Applications:|Suitable for use in Western Blot. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Source: Recombinant, E. coli|Purity: ~90% (SDS-PAGE). |Concentration: ~0.5mg/ml|Form: Supplied as a liquid in sodium phosphate, pH 7.2, (20mM), 150mM sodium chloride, 10% glycerol, 200mM Imidazole.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in sodium phosphate, pH 7.2, (20mM), 150mM sodium chloride, 10% glycerol, 200mM Imidazole.
Purity
~90% (SDS-PAGE).
References
1. Zho, J. (1998). Cell 94: 471-480 2. Boorstein, W. R., Ziegelhoffer, T. & Craig, E. A. (1993), J. Mol. Evol.38 (1) 1-17. 3. Rothman, J. (1989), Cell 59, 591 -601. 4. DeLuca-Flaherty et al. (1990), Cell 62, 875-887. 5. Bork, P., Sander, C. & Valencia, A. (1992), Proc. Nut1 Acad. Sci. USA 89, 7290-7294. |6. Fink, A.L. (1999) Physiol. Rev. 79: 425-449. 7. Smith, D.F., et al., (1993) Mol. Cell. Biol. 13(2):869-876. 8. Prapapanich, V., et al., (1996) Mol. Cell. Biol. 16(11):6200-6207. 9.Fernandez-Funez et al., (2000) Nature 408(6808):101-106.