Histone H3 K4me1/pT6 antibody
Referencia orb420388-50ug
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Histone H3 K4me1/pT6 antibody
Catalog Number: orb420388
| Catalog Number | orb420388 |
|---|---|
| Category | Antibodies |
| Description | Histone H3 K4me1/pT6 antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ChIP, DOT, IF, IHC, WB |
| Reactivity | C. elegans, Human |
| Isotype | IgG |
| Immunogen | Histone H3 [Monomethyl Lys4, p Thr6] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic monomethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 6 of human Histone H3.2. |
| Concentration | 0.55 mg/ml |
| Dilution range | ChIP: 2-5µg/million cells, IHC: 1:50, IF: 1:50, WB: 1:500 |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Anti-Histone H3 [Monomethyl Lys4, p Thr6] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined. |
| Conjugation | Unconjugated |
| UniProt ID | Q71DI3 |
| NCBI | NP_001005464 |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | rabbit anti-Histone H3 monomethyl Lys4 pT6 antibod |
| Note | For research use only |
| Application notes | Anti-Histone H3 [Monomethyl Lys4, p Thr6] antibody is tested for Western Blot, Immunofluorescence, and Dot Blot. This antibody is useful for Immunocytochemistry and Chromatin Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals. |
| Expiration Date | 12 months from date of receipt. |
Dot Blot of Rabbit Histone H3 [Monomethyl Lys4, p Thr6] Antibody. Lane 1: unmodified. Lane 2: pT6. Lane 3: K4Me1 pT6. Lane 4: K4Me2 pT6. Lane 5: K4Me3 pT6. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Monomethyl Lys4, p Thr6] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
Immunofluorescence of Rabbit Anti-Histone H3 [Monomethyl Lys4, p Thr6] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Monomethyl Lys4, p Thr6] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10000 for 45 min at RT. Localization: Histone H3 [Monomethyl Lys4, p Thr6] is nuclear and chromosomal. Staining: Histone H3 [Monomethyl Lys4, p Thr6] is expressed in green and the nuclei are counterstained with DAPI (blue).
Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys4, p Thr6] Antibody. Lane 1: C. elegans embryonic lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys4, p Thr6] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys4, p Thr6] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys4, p Thr6] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.