HMG1 (HMGB1) Mouse Monoclonal Antibody [Clone ID: 4C9]
Specifications
Product Data | |
Clone Name | 4C9 |
Applications | WB |
Recommended Dilution | Western blot: 1 μg/ml for chemiluminescence detection system. For details see protocol below. |
Reactivities | Human, Mouse, Rat |
Host | Mouse |
Isotype | IgG |
Clonality | Monoclonal |
Immunogen | Synthetic peptide corresponding to internal region of human HMGB1 |
Specificity | This antibody reacts with HMGB1. |
Formulation | PBS containing 50% glycerol, pH 7.2. No preservative is contained. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store (in aliqouts) at -20 °C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Gene Name | high mobility group box 1 |
Database Link | |
Background | High mobility group box 1 (HMGB1), named for its rapid migration properties on electrophoretic gels, is a member of the nonhistone chromatin-associated proteins. HMGB1 is translated as a 214 amino acid protein, and extensively modified posttranslationally, by glycosylation, acylation, methylation, and phosphorylation. The primary structure is evolutionarily conserved, with 100% amino acid sequence homology between rat and mouse, and 99% homology between rodent and human. Intracellular HMGB1 has been studied previously for its roles in binding DNA; stabilizing nucleosome formation; as a general transcription factor for nucleolar and mitochondrial RNA polymerases; and as a gene- and tissue-specific transcriptional regulator that can enhance transcription and/or replication. Extracellular HMGB1 is recently implicated as a late mediator of delayed endotoxin lethality, because murine and human macrophages/monocytes release large amounts of a 29 kDa form of HMGB1 when stimulated by exposure to bacterial endotoxin. |
Synonyms | High mobility group protein 1, HMG1, HMG-1, High mobility group protein B1, HMGB-1, Amphoterin |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting |
Reference Data |
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