Human ACPP (Acid Phosphatase, Prostatic) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	ACP3
Gene ID:	55
Uniprot ID:	PPAP_HUMAN
Sample Type:	serum, plasma, tissue homogenates or other biological fluids.

Tissue Specificity	Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Low expression in prostate carcinoma cells and tissues. Isoform 2: Widely expressed. Expressed in the sarcolemma of skeletal muscle.
Post Translational Modifications	N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides. Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection).
Function	A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Isoform 2: Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. PAPf39: (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection.
Protein Name	Prostatic Acid Phosphatase Pap 5'-Nucleotidase 5'-Nt Acid Phosphatase 3 Ecto-5'-Nucleotidase Protein Tyrosine Phosphatase Acp3 Thiamine Monophosphatase Tmpase Cleaved Into - Papf39
Database Links	Reactome: R-HSA-6798695
Cellular Localisation	Isoform 1: Secreted Isoform 2: Cell Membrane Single-Pass Type I Membrane Protein Lysosome Membrane Nucleus Cytoplasm Cytosol Appears To Shuttle Between The Cell Membrane And Intracellular Vesicles Colocalizes With Flot1 At Cell Membrane And In Intracellular Vesicles Colocalizes With Lamp2 On The Lysosome Membrane
Alternative ELISA Names	Prostatic Acid Phosphatase ELISA kit Pap ELISA kit 5'-Nucleotidase ELISA kit 5'-Nt ELISA kit Acid Phosphatase 3 ELISA kit Ecto-5'-Nucleotidase ELISA kit Protein Tyrosine Phosphatase Acp3 ELISA kit Thiamine Monophosphatase ELISA kit Tmpase Cleaved Into - Papf39 ELISA kit ACP3 ELISA kit ACPP ELISA kit
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Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance