Product Details | |
Product Name | Human IgG1 Matched Antibody Pair [RM117/RM129] Rabbit Monoclonal |
Product Description | The Human IgG1 Matched Antibody Pair recognize Human IgG1 specifically and does not cross-react to IgG from Non-Human Primate species (Rhesus and Cynomologus macaques), or mouse, rat, goat immunoglobulin. This Human IgG1 Matched Antibody Pair can be used for the detection of therapeutic antibodies from samples obtained from experimental animals to provide accurate pharmacokinetic (PK) data. |
Catalog No. | 31-1019-MK |
Clone Name | RM117 Anti-Human IgG1 Rabbit Monoclonal Antibody RM129 Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody |
Product Data Sheet | Human IgG1 Matched Antibody Pair |
Protocol | Sandwich ELISA Protocol |
Specificity | The antibody pair detects only human IgG1. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. There is no cross reactivity to any other immunoglobulins or IgG subclasses. |
Application | Sandwich ELISA, or other Sandwich Assays |
Species Reactivity | Human |
Conjugate | RM129: Biotin |
Intended Use | Research Use Only |
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Properties | |
Form | Liquid |
Storage Instructions | Store at -20.0°C |
Stability | Stable for 1 Year at -20.0°C from date of receipt |
Components | 1) Capture Antibody: Anti-Human IgG1 Rabbit Monoclonal antibody, clone RM117, 100 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 2) Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 3) 10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise. |
Purity | Protein A affinity purified from an animal origin–free culture supernatant |
Clone Type | Monoclonal |
Isotype | Rabbit IgG |
Keywords | |
Usage | ELISA: Capture Antibody 50 ng/well – 200 ng/well; Detection Antibody 0.1 µg/mL – 0.5 µg/mL |
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Product Images | |
Sandwich ELISA, using RevMAb human IgG1 matched antibody pair, shows species reactivity to human only, and shows no cross-reactivity to monkey (Cyno or Rhesus), mouse IgG, rat IgG, or goat IgG. | |
Detection of human IgG1 in monkey serum. Sandwich ELISA using RevMAb human IgG1 matched antibody pair, followed by a HRP conjugated streptavidin. | |
Detection of human IgG1 in mouse or rat serum. Sandwich ELISA using RevMAb human IgG1 matched antibody pair, followed by a HRP conjugated streptavidin. | |
Detection of human IgG1 in mouse or rat serum. Sandwich ELISA using RevMAb human IgG1 matched antibody pair, followed by a HRP conjugated streptavidin. |