Reactivos e instrumentos para inmunología, biología celular y biología molecular.
 
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Referencia NB-26-01358-50UG

embalaje : 50ug

Marca : Neo Biotech

Contact local distributor :


Teléfono : +1 850 650 7790

Product Details
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Dilutions: Immunohistochemistry: 5-10 ug/ml Immunofluorescence: 5-15 ug/ml Western Blot: 1 ug/ml
Immunogen: Synthetic peptide
Storage: The product can be stored as supplied for up to 12 months at 2°C-4°C. After reconstitution, aliquot and store at -20°C for higher stability or at 4°C with an appropriate antibacterial agent. Avoid freeze thaw-cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat
Entrez:
UniProt:
Immunohistochemistry

Immunohistochemical detection of Leptin in mouse hypothalamus. Mouse brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to Leptin at 15 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Cell nuclei were counterstained with DAPI (blue).

Immunohistochemistry

Immunohistochemical detection of Leptin in mouse hypothalamus. Mouse brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to Leptin at 15 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Cell nuclei were counterstained with DAPI (blue).

Western Blot

Western Blot