p27kip1, phosphorylated (Thr187) (Biotin)

The cyclin-dependent kinase inhibitor p27KIP1 is a potent inhibitor of both Cyclin E-Cdk2 and Cyclin A-Cdk2, two cdk complexes involved in the regulation of G1/S transition. p27 is phosphorylated on threonine 187 by cyclin E/Cdk2, and overexpression of this complex in mammalian cells induces degradation of transfected p27. The Cdk-mediated phosphorylation of p27 on threonine 187 is required for p27 ubiquitination and consequent degradation. The phosphorylation on threonine 187 and the ubiquitination of cellular p27, as well as p27 ubiquitinating activity, were found to be regulated collectively during the cell cycle, being present in proliferating cells but not in G0- and G1- arrested cells.||Applications:|Suitable for use in ELISA and Western Blot. Other applications have not been tested.||Recommended Dilution:|ELISA: 0.1-1ug/ml|Western Blot: 1-3ug/ml|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.