Rabbit Anti-Mouse Lambda Light Chain Secondary Antibody (AP)

Referencia LS-C60792-1

embalaje : 1mg

Marca : LSBio

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Teléfono : +1 850 650 7790

Overview

Antibody:
Mouse Lambda Light Chain Rabbit anti-Mouse Polyclonal (AP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Alkaline Phosphatase, Unmodified

Specifications

Description
Mouse Lambda Light Chain antibody LS-C60792 is an AP-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Target
Mouse Lambda Light Chain
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Alkaline Phosphatase
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Mouse lambda light chain.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), and anti-Rabbit Serum. Solid phase adsorption(s) against a panel of Mouse IgG kappa, IgM kappa and IgA kappa proteins was performed.  Specificity was confirmed by ELISA.. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Applications
  • IHC (1:200 - 1:1000)
  • Western blot (1:500 - 1:2500)
  • ELISA (1:2000)
Usage
Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate catalog no. NPP-10 as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

tech@clinisciences.com