Rabbit polyclonal antibody to Vinculin

Referencia CPA9462-200ul

embalaje : 200ul

Marca : Cohesion Biosciences

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Teléfono : +1 850 650 7790

  • Western blot analysis of Vinculin expression in Hela (A), K562 (B), mouse brain (C), rat brain (D) whole cell lysates.
  • Immunohistochemical analysis of Vinculin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-Vinculin Antibody
Cat No: CPA9462
Source: Rabbit
Reactivity: H, M, R
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Description: Rabbit polyclonal antibody to Vinculin
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Vinculin. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500)
Gene Symbol: VCL
Alternative Names: Vinculin; Metavinculin; MV
Entrez Gene (Human):
Entrez Gene (Mouse) :
Entrez Gene (Rat) :
SwissProt (Human):
SwissProt (Mouse) :
SwissProt (Rat) :
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.