SiO2 with natural hydroxyl or silanol (SiOH) surface groups for adsorption of nucleotides with low protein binding. Non-functionalized silica microspheres are available as ~10% solids (w/w) aqueous suspensions or in the dry form in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g.
Silica in aqueous suspension
TechNote 104, Silica Microspheres
PDS 702, Silica Microspheres
SDS SG140 (Silica in Suspension)
| Catalog Number | Nominal Diameter | Specification Range |
|---|---|---|
| SS02000 | 0.15µm | 0.120 - 0.180µm |
| SS02001 | 0.30µm | 0.260 - 0.340µm |
| SS02002 | 0.40µm | 0.360 - 0.440µm |
| SS03000 | 0.50µm | 0.460 - 0.540µm |
| SS03001 | 0.70µm | 0.660 - 0.740µm |
| SS03002 | 0.90µm | 0.860 - 0.940µm |
| SS04000 | 1.0µm | 0.95 - 1.05µm |
| SS04001 | 1.50µm | 1.40 - 1.60µm |
| SS04002 | 2.0µm | 1.80 - 2.20µm |
| SS05000 | 2.50µm | 2.30 - 2.70µm |
| SS05001 | 3.0µm | 2.80 - 3.20µm |
| SS05002 | 4.0µm | 3.8 - 4.20µm |
| SS05003 | 5.0µm | 4.80 - 5.20µm |
Dry Silica
TechNote 104, Silica Microspheres
PDS 702, Silica Microspheres
SDS SG141 (Dry Silica)
| Catalog Number | Nominal Diameter | Specification Range |
|---|---|---|
| SSD2001 | 0.30µm | 0.260 - 0.340µm |
| SSD3000 | 0.50µm | 0.460 - 0.540µm |
| SSD4000 | 1.0µm | 0.95 - 1.05µm |
| SSD4001 | 1.50µm | 1.40 - 1.60µm |
| SSD5000 | 2.50µm | 2.30 - 2.70µm |
| SSD5001 | 3.0µm | 2.80 - 3.20µm |
| SSD5002 | 4.0µm | 3.80 - 4.20µm |
| SSD5003 | 5.0µm | 4.80 - 5.20µm |
References
Ferguson JA, Steemers FJ, Walt DR. (2000) High-density fiber-optic DNA random microsphere array. Anal Chem; 72(22):5618-5624.
Lauer S, Goldstein B, Nolan RL, Nolan JP. (2002) Analysis of cholera toxin-ganglioside interactions by flow cytometry. Biochemistry; 41(6):1742-51.
Steinberg G, Stromsborg K, Thomas L, Barker D, Zhao C. (2004) Strategies for covalent attachment of DNA to beads. Biopolymers; 73(5):597-605.
B Sharma, P Bugga, L Madison, A Henry, M Blaber, N Greeneltch, ... R. Van Duyne (2016) Bisboronic Acids for Selective, Physiologically Relevant Direct Glucose Sensing with Surface-Enhanced Raman Spectroscopy Am Chem Soc, 138 (42), pp 13952–13959
G Steinberg-Tatman,M Huynh, D Barker, C Zhao (2006) Synthetic Modification of Silica Beads That Allows for Sequential Attachment of Two Different Oligonucleotides Bioconjugate Chem 17(3), 841-848
N Clack, K Salaita, J Groves (2008) Electrostatic readout of DNA microarrays with charged microspheres. Nat Biotech 26,825-830
Lu Q, Liu H, Cao T (2013) Efficient Isolation of Bone Marrow Adipocyte Progenitors by Silica Microbeads Incubation. Stem Cells Dev. V22:18
Khan Z, Poetter K, Park DJ.(2008) Enhanced solid phase PCR: mechanisms to increase priming by solid support primers. Anal Biochem. Apr 15;375(2):391-3.
Marrec P, Doglioli M, Grégori G, Dugenne M, Della Penna A, Bhairy N, … Thyssen M (2017) Coupling physics and biogeochemistry thanks to high resolution observations of the phytoplankton community structure in the North-Western Mediterranean Sea Biogeosciences Discuss, https://doi.org/10.5194/bg-2017-343 (0.4µm - 7µm silica microspheres as FSC standards for phytoplankton on the CytoBuoy CytoSense)
Wang, Z. (2016). Microsphere super-resolution imaging. Nanoscience, 3, 193-210. (4.7µm silica microspheres)
Crites, T. J., Maddox, M., Padhan, K., Muller, J., Eigsti, C., & Varma, R. (2015). Supported lipid bilayer technology for the study of cellular interfaces. Current protocols in cell biology, 24-5. (lipid bilayers [aAPCs, artificial antigen-presenting cells] on 5µm silica microspheres)
Zanoni, M., Habimana, O., Amadio, J., & Casey, E. (2016). Antifouling activity of enzyme‐functionalized silica nanobeads. Biotechnology and bioengineering, 113(3), 501-512.(500nm silica microspheres, functionalized & coated with Proteinase K for biofilm / biofouling control)
Tolić-Nørrelykke, SF, Schäffer, E, Howard, J, Pavone, FS, Jülicher, F., & Flyvbjerg, H. (2006) Calibration of optical tweezers with positional detection in the back focal plane. Review of scientific instruments, 77(10), 103101.(SS04001, 1.54µm silica microspheres; PS03001, 0.523µm polystyrene microspheres)
Bhebhe N, Williams PAC, Rosales-Guzmán C, Rodriguez-Fajardo V, Forbes A. (2018) A vector holographic optical trap. Sci.Rep. Vol. 8:#17387 (Holographic optical tweezers with arrays of HOPS beams demonstrated using 2µm silica microspheres)