Tubulin, alpha (BSA & Azide Free) (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1) (Biotin)

Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.||Applications:|Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.||Recommended Dilutions:|Western Blot: 1-2ug/ml for 2hrs at RT|Immunohistochemistry (Formalin/paraffin): 1:400 for 20 minutes at RT. No special pretreatment is requiredfor stainingof formalin-fixed, paraffin-embedded tissues.|Immunoprecipitation (Native and denatured): 2ug/mg protein lysate using Protein G.|Optimal dilutions to be determined by the researcher.||Positive Controls: |LS174T, MAD109 cells, skin and lung||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.