β-Galactosidase Reporter Assay Kit
Cat# K2183-200
Size : 200T
Marca : APExBIO Technology
Contatta il distributore locale :
Telefono : +1 850 650 7790
β-Galactosidase Reporter Assay Kit
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β-Galactosidase Reporter Assay Kit is a kit for the detection of the β-galactosidase reporter gene using colorless ONPG as substrates. β-galactosidase is a commonly used reporter molecule that can be used to transfect cells with the luciferase reporter and serve as an internal control for the luciferase reporter, thereby eliminating experimental errors due to differences in transfection efficiency. The substrate provided in this kit produces a yellow product catalyzed by β-galactosidase, and the activity of β-galactosidase can be determined by measuring the absorbance at 420 nm. The lysis buffer provided in this kit is suitable for the lysis of animal cells, plant cells or tissue samples.
Figure 1: The standard curve for this kit
Protocol| Components | K2183-200 T |
| Lysis Buffer | 100 mL |
| Assay Reagent | 10 mL |
| Stop Buffer | 35 mL |
| Store the kit at -20°C, stable for at least 6 months. Assay Reagent should be stored at -20°C away from light. | |