β-Glucosidase Assay Kit, BioAssay™

b-Glucosidase is a glucosidase enzyme which acts upon beta1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). b-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a b-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a b-glucosidase isoform (lysosomal glucocerebrosidase) has been related to Gaucher's disease and Parkinson's disease. This b-Glucosidase Assay Kit is designed to measure b-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-b-D-glucopyranoside that is hydrolyzed specifically by b-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity. ||Key Features:|High sensitivity and wide linear range. Use 20ul sample. The detection limit is 2U/L, linear up to 250U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of b-glucosidase activity within 20 minutes. Robust and amenable to high throughput analysis.||Applications:|Direct Assays: b-glucosidase activity in biological samples. Characterization and Quality Control for b-glucosidase production. Drug Discovery: high-throughput screen for b-glucosidase modulators. ||Unit Definition: |One unit of enzyme catalyzes the hydrolysis of 1mmole of substrate per min at pH 7.0.||Kit Components (100 tests in 96-well plates):|G3059-11A: Assay Buffer, pH 7.0, 1x24ml |G3059-11B: b-NPG Substrate, 1x1ml |G3059-11C: Calibrator, 1x10ml (equivalent to 250 U/L) ||Storage and Stability:|Store all components at -20°C. Stable for 6 months after receipt.