2’-Deoxythymidine-5’-Triphosphate, Sodium Salt (dTTP) Solution, BioGenomics™

PCR Grade nucleotides are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).||Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.||dTTP, Molecular Biology Grade, is a 100mM clear, colorless solution of the sodium salt (pH 8.3).||Synonyms:|Thymidine 5'-(Tetrahydrogen Triphosphate) Sodium Salt; Thymidine-5’-Triphosphate, Sodium Salt; dTTP; 2'-Deoxythymidine 5'-triphosphate trisodium salt||Usage:|250ul at 100mM = 6,250 standard reactions|1,250ul at 100mM = 31,250 standard reactions||CAS No: |18423-43-3|Free Acid: 365-08-2||Purity:|≥99% (HPLC)||Appearance:|Supplied as a liquid||dNDP:|≤0.9%||pH:|8.3||Quality Control:|Functionally tested with Sequenase, Taq DNA Polymerase, T7 DNA Polymerase and Pfu DNA Polymerase. ||Concentration:|100mM||Lambda Max:|267±2nm||A250/A260:|0.65 ± 0.03||A280/A260:|0.73 ± 0.03||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.||™The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche.