4-Azido-L-phenylalanine (hydrochloride) [34670-43-4]
Cat# HY-16714A-100mg
Size : 100mg
Marca : MedChemExpress
4-Azido-L-phenylalanine hydrochloride is an unnatural amino acid, which is used as an effective vibrational reporter of local protein environments. 4-Azido-L-phenylalanine (hydrochloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.
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4-Azido-L-phenylalanine hydrochloride Chemical Structure
CAS No. : 34670-43-4
This product is a controlled substance and not for sale in your territory.
Based on 7 publication(s) in Google Scholar
Other Forms of 4-Azido-L-phenylalanine hydrochloride:
- 4-Azido-L-phenylalanine In-stock
Description |
4-Azido-L-phenylalanine hydrochloride is an unnatural amino acid, which is used as an effective vibrational reporter of local protein environments. 4-Azido-L-phenylalanine (hydrochloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups. |
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In Vitro |
In cell-free protein synthesis (CFPS) method, 0.9-1.7 mg/mL of modified soluble super-folder green fluorescent protein (sfGFP) containing either 4-Azido-L-phenylalanine or p-propargyloxy-l-phenylalanine (pPaF) accumulate in the CFPS solutions[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Masse moléculaire |
242.66 |
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Formule |
C9H11ClN4O2 |
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CAS No. |
34670-43-4 |
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Appearance |
Solid |
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Color |
Off-white to light yellow |
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SMILES |
O=C(O)[C@@H](N)CC1=CC=C(N=[N+]=[N-])C=C1.Cl |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
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Solvant et solubilité |
In Vitro:
DMSO : ≥ 103.3 mg/mL (425.70 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : 10 mg/mL (41.21 mM; Need ultrasonic) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureté et documentation |
Purity: 99.88% |
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Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O / DMSO | 1 mM | 4.1210 mL | 20.6050 mL | 41.2099 mL | 103.0248 mL |
5 mM | 0.8242 mL | 4.1210 mL | 8.2420 mL | 20.6050 mL | |
10 mM | 0.4121 mL | 2.0605 mL | 4.1210 mL | 10.3025 mL | |
15 mM | 0.2747 mL | 1.3737 mL | 2.7473 mL | 6.8683 mL | |
20 mM | 0.2060 mL | 1.0302 mL | 2.0605 mL | 5.1512 mL | |
25 mM | 0.1648 mL | 0.8242 mL | 1.6484 mL | 4.1210 mL | |
30 mM | 0.1374 mL | 0.6868 mL | 1.3737 mL | 3.4342 mL | |
40 mM | 0.1030 mL | 0.5151 mL | 1.0302 mL | 2.5756 mL | |
DMSO | 50 mM | 0.0824 mL | 0.4121 mL | 0.8242 mL | 2.0605 mL |
60 mM | 0.0687 mL | 0.3434 mL | 0.6868 mL | 1.7171 mL | |
80 mM | 0.0515 mL | 0.2576 mL | 0.5151 mL | 1.2878 mL | |
100 mM | 0.0412 mL | 0.2060 mL | 0.4121 mL | 1.0302 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.