Alkaline Phosphatase (ALP) Assay Kit is an assay kit designed for the detection and quantification of Alkaline Phosphatase activity in serum, plasma, tissue homogenates and cell lysates using a microplate reader. Alkaline phosphatase decomposes benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form a red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value at 520 nm.
Research Articles on Alkaline Phosphatase (ALP)
| Target | Alkaline Phosphatase (ALP) |
| Reactivity | General (All species) |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Validity | The validity for this kit is 12 months. |
| Test Range | 0.13 King Unit/100 ml - 50 King Unit/100 ml |
| Sensitivity | 0.13 King Unit/100 ml |
| Detection Method | Colorimetric |
| Assay Data | Quantitative |
| Sample Type | Serum, plasma, tissue homogenates and cell lysates. |
| CAS Number | 9001-78-9 |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |