K2216-100 assays | Alpha-Ketoglutarate Colorimetric/Fluorometric Assay

Background

α-Ketoglutarate (α-KG) is a keto acid and is an important intermediate in the Krebs cycle, locating after isocitrate and before succinyl CoA. Anaplerotic reactions can replenish the Krebs cycle by synthesizing α-KG from glutamate through the action of glutamate dehydrogenase or from transamination of glutamate. α-KG is also an important nitrogen transporter. Measurement of α-KG in newborns is an indicator of inborn errors of metabolism.

The Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit provides a highly sensitive, fast and convenient way for detection of α-KG levels in various biological samples based on colorimetric and fluorometric method. In the assay, α-KG is transaminated with the production of pyruvate which is utilized to transform a nearly colorless probe to both fluorescence (Ex/Em = 535/587 nm) and color (λmax = 570 nm) product. The kit can detect α-KG in the range of 0.01 to 10 nmoles.

Features & Properties

Features	Fast and convenient; Simple procedure; Takes approx. 30 minutes to complete the assay; Highly sensitive assays for measuring alpha-Ketaglutarate levels in biological samples.
Shipping	Blue Ice
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

α-KG Assay Buffer, α-KG OxiRed Probe (in DMSO), α-KG Converting Enzyme, α-KG Development Enzyme Mix, α-KG Standard (10 µmol)

Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit

Cat# K2216-100 assays

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