AP2509A-ev | Androgen Receptor (ANDR) Antibody Clinisciences

ANDR (Cat. #AP2509a) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the AR antibody detected the AR protein (arrow).
ANDR Antibody (Sumo-site) (Cat. #AP2509a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the ANDR antibody detected the ANDR protein (arrow).
Androgen Receptor Antibody (ANDR) (Cat. #AP2509a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Androgen Receptor Antibody (ANDR) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	IHC-P, WB, E
Primary Accession	P10275
Other Accession	Q9GKL7, P19091, O97952, Q5JUN9
Reactivity	Human, Mouse
Predicted	Monkey, Pig
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	99188 Da
Antigen Region	365-392 aa

Additional Information
Gene ID	367
Other Names	Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AR, DHTR, NR3C4
Target/Specificity	This Androgen Receptor (ANDR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-392 amino acids from human Androgen Receptor (ANDR).
Dilution	WB~~1:1000 IHC-P~~1:10~50
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Androgen Receptor (ANDR) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	AR
Synonyms	DHTR, NR3C4
Function	Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
Cellular Location	Nucleus. Cytoplasm Note=Detected at the promoter of target genes (PubMed:25091737) Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.
Tissue Location	[Isoform 2]: Mainly expressed in heart and skeletal muscle.

Androgen Receptor (ANDR) Antibody