Protocol

1. Bring all reagents to room temperature before use.
2. Use a pipette to remove excess liquid on the sample slide. Allow the slide to air dry slightly.
3. Add 20 µl of Anti-Fade Mounting Medium on to the sample on the slide, or add an appropriate volume of Anti-Fade Mounting Medium in to the cell culture wells directly.
4. Place a coverslip on the slide. Take care to avoid bubble formation.
5. Observe under a fluorescence microscope. The coverslipped slide can be stored in the dark at 4 °C or at -20 °C for up to 3 weeks without fluorescence fading.

Notes:

• Coverslips which contain Anti-Fade Mounting Medium must be stored in the dark.
• This medium can help to slow down the rate of quenching, but does not eliminate it. It is therefore recommended to observe the slide and capture images as soon as possible.
• Wear Personal Protective Equipment (PPE), such as lab coats, lab glasses and gloves, when using this product.