General Info
| Host: | Mouse |
| Applications: | WB/ICC |
| Reactivity: | Human/Mouse/Rat/Monkey/Hamster |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Mouse monoclonal antibody anti-Flap endonuclease 1 is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: | Monoclonal |
| Clone ID: | 7H8-F4-C11 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000ICC 1:400 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
| Gene Symbol: | FEN1 |
| Gene ID: | 2237 |
| Uniprot ID: | FEN1_HUMAN |
| Specificity: | This antibody detects endogenous levels of FEN-1 and does not cross-react with related proteins. |
| Immunogen: | Purified recombinant human FEN-1 protein fragments expressed in E.coli. |
Description
| Function | Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. |
| Protein Name | Flap Endonuclease 1Fen-1Dnase IvFlap Structure-Specific Endonuclease 1Maturation Factor 1Mf1Hfen-1 |
| Database Links | Reactome: R-HSA-110362Reactome: R-HSA-162594Reactome: R-HSA-174437Reactome: R-HSA-5651801Reactome: R-HSA-5685939Reactome: R-HSA-69166 |
| Cellular Localisation | Isoform 1: NucleusNucleolusNucleusNucleoplasmResides Mostly In The Nucleoli And Relocalizes To The Nucleoplasm Upon Dna DamageIsoform Fenmit: Mitochondrion |
| Alternative Antibody Names | Anti-Flap Endonuclease 1 antibodyAnti-Fen-1 antibodyAnti-Dnase Iv antibodyAnti-Flap Structure-Specific Endonuclease 1 antibodyAnti-Maturation Factor 1 antibodyAnti-Mf1 antibodyAnti-Hfen-1 antibodyAnti-FEN1 antibodyAnti-RAD2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance