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> Anti-Human IgG IgA IgM (H&L) (Fluorescein Conjugated) Secondary Antibody

Anti-Human IgG IgA IgM (H&L) (Fluorescein Conjugated) Secondary Antibody

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Cat# ASR1918

Size : 2mg

Marca : Abcepta

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  • DB - Anti-Human IgG IgA IgM (H&L) (Fluorescein Conjugated) Secondary Antibody ASR1918
    Dot Blot of Goat anti-Human IgG IgA IgM Antibody Fluorescein Conjugated. Antigen: Row 1 - Human IgG Row 2 - Human IgA Row 3 - Human IgM. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Human IgG IgA IgM Antibody Fluorescein Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.
Product Information
Description Anti-HUMAN IgG IgA IgM (H&L) (GOAT) Antibody Fluorescein Conjugated
Host Goat
Conjugate Fluorescein (FITC)
FP Value 5.1 moles Fluorescein (FITC) per mole of IgG
Target Species Human
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, FC
Application Note FLISA 1:10,000-1:50,000
IF Microscopy 1:1,000-1:5,000
FlowCytometry 1:500-1:2,500
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG IgA IgM
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Human IgG, IgA and IgM whole molecules
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG, Human IgA and Human IgM.  This reagent is suitable for the detection of all Human isotypes and chain combinations.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.