Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Anti-Kv4.3 K+ Channel Antibody

Stampa

Cat# 75-017

Size : 100uL

Contatta il distributore locale :

Telefono : +1 850 650 7790

Marca : Antibodies Incorporated

Richiedere ulteriori informazioni

Contatta il distributore locale :

Telefono : +1 850 650 7790

Species Reactivity

Drosophila, Hamster, Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Data Sheet

KO Validated

SKU: 75-017

Product Details

Target

Kv4.3 K+ channel

Target Description

Kv4.3 K+ channel (Potassium voltage-gated channel subfamily D member 3 ) is a member of the mammalian Shal-related family and the potassium voltage gated channel subunit family. Kv4 subunit family includes four Kv4 α subunits (Kv4.1, Kv4.2 and Kv4.3). However only Kv4.2 and Kv4.3 are predominantely epressed in the brain (Alfaro-Ruiz et al., 2005). KV4.3 subunit is encoded by gene KCND3 in human. It has been demonstrated that Kv 4.2 and Kv4.3 are often colocalized with pain-modulating molecules in rat spinal lamina II excitatory interneurons (Huang et al., 2005).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K75/41

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Kcnd3

Molecular Weight

75 kDa in rat brain membrane preparations

Antigen

Fusion protein amino acids 415-636 (cytoplasmic C- terminus) of rat Kv4.3 (accession number Q62897) produced recombinantly in E. Coli

Species Reactivity

Drosophila, Hamster, Human, Mouse, Rat

Antibody Registry ID

AB_2131966

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity against rat Kv4.2 expressed in transfected cells.

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Potassium voltage-gated channel subfamily D member 3 (Voltage-gated potassium channel subunit Kv4.3)

UniProt Details

UniProt (Human): Q9UK17
UniProt (Immunogen Species): Q62897

Citations (98)

Product Specific References for Applications and Species

Array Tomography: Rat
HIstoblotting: Mouse
Immunocytochemistry: Human | Mouse | Rat
Immunohistochemistry: Mouse | Rat
Immunoprecipitation: Human | Mouse
Western Blot: Drosophila | Guinea Pig | Human | Mouse | Non-Human Primate | Rabbit| Rat
Additional Publications: Unspecified

Array Tomography: Rat
PMID	Dilution	Publication
26400949	1:600	Simkin, D., et al. 2015. Aging-related hyperexcitability in CA3 pyramidal neurons is mediated by enhanced A-type K+ channel function and expression. . Journal of Neuroscience, 13206-13218.

Histoblotting: Mouse
PMID	Dilution	Publication
30634540	not listed	Alfaro-Ruíz, R., et al. 2019. Expression, cellular and subcellular localisation of Kv4. 2 and Kv4. 3 channels in the rodent hippocampus. . International journal of molecular sciences, 246.

Immunocytochemistry: Human
PMID	Dilution	Publication
36097791	1:400	Chiu, C.Y., et al. 2022. K+ Channel Kv4.1 is Expressed in the Nociceptors/Secondary Nociceptive Neurons and Participates in Pain Regulation. European Journal of Pain, 2238-2256.
30588629	not listed	Tyan, L., et al. 2019. Long QT syndrome caveolin‐3 mutations differentially modulate Kv4 and Cav1. 2 channels to contribute to action potential prolongation. . The Journal of physiology, 1531-1551.
23324056	1:5 (supe)	David, J.P., et al. 2013. Trafficking of the IKs -complex in MDCK cells: site of subunit assembly and determinants of polarized localization.. Traffic, 399-411.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
31064843	not listed	Lubbers, E.R., et al. 2019. Defining new mechanistic roles for αii spectrin in cardiac function. . Journal of Biological Chemistry, 9576-9591.
29802197	1:10 (supe)	Cheng, L., et al. 2018. Coupling of Smoothened to inhibitory G proteins reduces voltage-gated K+ currents in cardiomyocytes and prolongs cardiac action potential duration. . Journal of Biological Chemistry, 11022-11032.
29802197	1:10 (supe)	Cheng, L., et al. 2018. Coupling of Smoothened to inhibitory G proteins reduces voltage-gated K+ currents in cardiomyocytes and prolongs cardiac action potential duration. . Journal of Biological Chemistry, 11022-11032.
26196737	1:100	Liu, J., et al. 2015. Kv4.3-Encoded Fast Transient Outward Current Is Presented in Kv4.2 Knockout Mouse Cardiomyocytes.. PLoS One, e0133274.
21507952	1:10 (supe)	Cheng, L., et al. 2011. Cortactin is required for N-cadherin regulation of Kv1.5 channel function.. The Journal of Biological Chemistry, 20478-20489.

Immunocytochemistry: Rat
PMID	Dilution	Publication
26239200	1:8000	Cheng, C.F., et al. 2016. Coexpression of auxiliary subunits KChIP and DPPL in potassium channel Kv4-positive nociceptors and pain-modulating spinal interneurons.. Journal of Comparative Neurology, 846-73.
26016905	1:200	You, T., et al. 2015. Two novel Brugada syndrome-associated mutations increase KV4.3 membrane expression and function.. International Journal of Molecular Medicine, 309-315.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
38942263	1:1000	Esaki, H, et al. 2024. Role of medial prefrontal cortex voltage-dependent potassium 4.3 channels in nicotine-induced enhancement of object recognition memory in male mice. European journal of pharmacology, 176790.
36640913	1:500	Otuyemi, B., et al. 2023. Domain and Cell Type-specific Immunolocalisation of Voltage-gated Potassium Channels in the Mouse Striatum. Journal of Chemical Neuroanatomy, 102233.
36097791	1:400	Chiu, C.Y., et al. 2022. K+ Channel Kv4.1 is Expressed in the Nociceptors/Secondary Nociceptive Neurons and Participates in Pain Regulation. European Journal of Pain, 2238-2256.
35098924	1:1500	Hobson, B.D., et al. 2022. Subcellular proteomics of dopamine neurons in the mouse brain. eLife, e70921.
31248728	1:250	Zheng, Y., et al. 2019. Deep sequencing of somatosensory neurons reveals molecular determinants of intrinsic physiological properties. . Neuron, 598-616.
31248728	1:250	Zheng, Y., et al. 2019. Deep sequencing of somatosensory neurons reveals molecular determinants of intrinsic physiological properties. . Neuron, 598-616.
30634540	not listed	Alfaro-Ruíz, R., et al. 2019. Expression, cellular and subcellular localisation of Kv4. 2 and Kv4. 3 channels in the rodent hippocampus. . International journal of molecular sciences, 246.
22612819	not listed	Menegola, M., et al. 2012. The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice.. Epilepsia, 142-149.
21676499	0.25	Wykes, R., et al. 2012. Changes in the physiology of CA1 hippocampal pyramidal neurons in preplaque CRND8 mice. . Neurobiology of aging, 1609-1623.
21329741	1:500	Prüss, H., et al. 2011. Potassium channel expression in adult murine neural progenitor cells.. Neuroscience, 19-29.
17122053	1:1000	Burkhalter, A., et al. 2006. Differential expression of I(A) channel subunits Kv4.2 and Kv4.3 in mouse visual cortical neurons and synapses.. Journal of Neuroscience, 12274-82.
17122038	not listed	Menegola, M., et al. 2006. Unanticipated region- and cell-specific downregulation of individual KChIP auxiliary subunit isotypes in Kv4.2 knock-out mouse brain.. Journal of Neuroscience, 2137-42.

Immunohistochemistry: Rat
PMID	Dilution	Publication
36181963	1:400	Chiu, C.Y., et al. 2022. NS5806 Inhibits ERK Activation to Attenuate Pain Induced by Peripheral Nerve Injury. Neuroscience Letters, 136890.
32899153	not listed	Alfaro-Ruíz, R., et al. 2020. Cellular and subcellular localisation of kv4-associated kchip proteins in the rat cerebellum. . International journal of molecular sciences, 6403.
32726631	not listed	Holderith, N., et al. 2020. A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses. Cell Reports, 107968.
32490811	1:500	Oláh, V.J., et al. 2020. Functional specification of CCK+ interneurons by alternative isoforms of Kv4. 3 auxiliary subunits. . Elife, e58515.
28330877	1:4000	Kuo, Y., et al. 2017. K + Channel Modulatory Subunits KChIP and DPP Participate in Kv4-Mediated Mechanical Pain Control. Journal of Neuroscience, 4391-4404.
27807163	1:500	Rizwan, A.P., et al. 2016. Long-Term potentiation at the mossy fiber–granule cell relay invokes postsynaptic second-messenger regulation of Kv4 channels..  Journal of Neuroscience, 11196-11207.
26239200	1:4000	Cheng, C.F., et al. 2016. Coexpression of auxiliary subunits KChIP and DPPL in potassium channel Kv4-positive nociceptors and pain-modulating spinal interneurons.. Journal of Comparative Neurology, 846-73.
26400949	1:500	Simkin, D., et al. 2015. Aging-related hyperexcitability in CA3 pyramidal neurons is mediated by enhanced A-type K+ channel function and expression. . Journal of Neuroscience, 13206-13218.
25355692	1:100-1:1000	Wang, W.C., et al. 2015. Immunohistochemical localization of DPP10 in rat brain supports the existence of a Kv4/KChIP/DPPL ternary complex in neurons.. Journal of Comparative Neurology, 608-628.
24037673	not listed	Rainnie, D.G., et al. 2014. Distribution and functional expression of Kv4 family α subunits and associated KChIP β subunits in the bed nucleus of the stria terminalis.. Journal of Comparative Neurology, 609-25.
22820170	1:200	Matsuyoshi, H., et al. 2012. Distinct cellular distributions of Kv4 pore-forming and auxiliary subunits in rat dorsal root ganglion neurons.. Life Sciences, 258-263.
22473424	1:100	Huang, C.Y., et al. 2012. Coexpression of high-voltage-activated ion channels Kv3.4 and Cav1.2 in pioneer axons during pathfinding in the developing rat forebrain.. Journal of Comparative Neurology, 3650-3672.
21451062	not listed	Guan, D., et al. 2011. Postnatal development of A-type and Kv1- and Kv2-mediated potassium channel currents in neocortical pyramidal neurons.. Journal of Neurophysiology, 2976-88.
21129448	1:1000	Bourdeau, M.L., et al. 2011. KChIP1 modulation of Kv4.3-mediated A-type K(+) currents and repetitive firing in hippocampal interneurons.. Neuroscience, 173-87.
20849929	1:1000	Dabrowska, J., et al. 2010. Expression and distribution of Kv4 potassium channel subunits and potassium channel interacting proteins in subpopulations of interneurons in the basolateral amygdala.. Neuroscience, 721-33.
18727953	not listed	Monaghan, M.M., et al. 2008. Altered expression and localization of hippocampal A-type potassium channel subunits in the pilocarpine-induced model of temporal lobe epilepsy.. Neuroscience, 550-562.
18495361	1:500	Menegola, M., et al. 2008. Dendritic A-type potassium channel subunit expression in CA1 hippocampal interneurons.. Neuroscience, 953-64.
18495361	not listed	Menegola, M., et al. 2008. Dendritic A-type potassium channel subunit expression in CA1 hippocampal interneurons.. Neuroscience, 953-64.
16525047	1:500	Kollo, M., et al. 2006. Novel subcellular distribution pattern of A-type K+ channels on neuronal surface. Journal of Neuroscience, 2684-91.

Immunoprecipitation: Human
PMID	Dilution	Publication
22198508	0.8 ug	Zhang, Y.H., et al. 2012. Modulation of human cardiac transient outward potassium current by EGFR tyrosine kinase and Src-family kinases.. Cardiovascular Research, 424-433.

Immunoprecipitation: Mouse
PMID	Dilution	Publication
38891076	12ug	Belghazi, M, et al. 2024. High-Resolution Proteomics Unravel a Native Functional Complex of Cav1.3, SK3, and Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels in Midbrain Dopaminergic Neurons. Cells, .
31064843	2ug	Lubbers, E.R., et al. 2019. Defining new mechanistic roles for αii spectrin in cardiac function. . Journal of Biological Chemistry, 9576-9591.

Western Blot: Drosophila
PMID	Dilution	Publication
34932577	1:500	Vallejos, M.J., et al. 2021. Age-related changes in Kv4/Shal and Kv1/Shaker expression in Drosophila and a role for reactive oxygen species. PloS One, e0261087.

Western Blot: Guinea Pig
PMID	Dilution	Publication
26764482	1:500	Nassal, D.M., et al. 2016. Myocardial KChIP2 expression in guinea pig resolves an expanded electrophysiologic role. . PloS one, e0146561.

Western Blot: Human
PMID	Dilution	Publication
31074016	1:1000	Wang, Y., et al. 2019. Contribution of potassium channels to action potential repolarization of human embryonic stem cell‐derived cardiomyocytes. . British Journal of Pharmacology, 2780-2794.
25854634	1:1000	Duarri, A., et al. 2015. Spinocerebellar ataxia type 19/22 mutations alter heterocomplex Kv4.3 channel function and gating in a dominant manner.. Cell Mol Life Sciences, 3387-3399.
22160437	not listed	Marshall, G.E., et al. 2011. Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade.. Pflügers Archiv: European Journal of Physiology, 537-548.

Western Blot: Mouse
PMID	Dilution	Publication
36842733	not listed	Markandeya, Y.S., et al. 2023. Caveolin-3 and Caveolae Regulate Ventricular Repolarization. Journal of Molecular and Cellular Cardiology, 38-49.
32822706	not listed	Tiwari, D., et al. 2020. The potassium channel Kv4. 2 regulates dendritic spine morphology, electroencephalographic characteristics and seizure susceptibility in mice.. Experimental Neurology, 113437.
31064843	not listed	Lubbers, E.R., et al. 2019. Defining new mechanistic roles for αii spectrin in cardiac function. . Journal of Biological Chemistry, 9576-9591.
27023865	not listed	Schilling, J.M., et al. 2016. Electrophysiology and metabolism of caveolin-3-overexpressing mice. . Basic research in cardiology, 1-13.
27023865	not listed	Schilling, J.M., et al. 2016. Electrophysiology and metabolism of caveolin-3-overexpressing mice. . Basic research in cardiology, 1-13.
26611208	not listed	Zhang, X., et al. 2016. Persistent increases in Ca(2+) influx through Cav1.2 shortens action potential and causes Ca(2+) overload-induced afterdepolarizations and arrhythmias.. Basic Research in Cardiology, 1-13.
24375641	1:200	Lopez-Izquierdo, A., et al. 2014. The absence of insulin signaling in the heart induces changes in potassium channel expression and ventricular repolarization.. American Journal of Physiology-Heart and Circulatory Physiology, H747-H754.
21943606	1:1000	Sun, W., et al. 2011. DPP6 establishes the A-type K(+) current gradient critical for the regulation of dendritic excitability in CA1 hippocampal neurons.. Neuron, 1102-15.
21646717	not listed	Stein, A.B., et al. 2011. Loss of H3K4 methylation destabilizes gene expression patterns and physiological functions in adult murine cardiomyocytes.. Journal of Clinical Investigation, 2641-2650.
21507952	not listed	Cheng, L., et al. 2011. Cortactin is required for N-cadherin regulation of Kv1.5 channel function.. The Journal of Biological Chemistry, 20478-20489.
20943905	not listed	Norris, A.J., et al. 2010. Interdependent roles for accessory KChIP2, KChIP3, and KChIP4 subunits in the generation of Kv4-encoded IA channels in cortical pyramidal neurons.. Journal of Neuroscience, 13644-13655.
18045613	not listed	Niwa, N., et al. 2008. Kv4.3 is not required for the generation of functional Ito,f channels in adult mouse ventricles.. Journal of Molecular and Cellular Cardiology, 95-104.

Western Blot: Rabbit
PMID	Dilution	Publication
22160437	not listed	Marshall, G.E., et al. 2011. Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade.. Pflügers Archiv: European Journal of Physiology, 537-548.

Western Blot: Rat
PMID	Dilution	Publication
29881800	not listed	Lai, Y.C., et al. 2018. Myocardial remodeling and susceptibility to ventricular tachycardia in a model of chronic epilepsy. . Epilepsia open, 213-223.
28677679	1:100	Sakai, A., et al. 2017. MicroRNA cluster miR-17-92 regulates multiple functionally related voltage-gated potassium channels in chronic neuropathic pain. . Nature communications, 1-13.
28263709	1:500	Nassal, D.M., et al. 2017. KChIP2 is a core transcriptional regulator of cardiac excitability.. Elife, 17304.
27259067	1:1000	Lei, Z., et al. 2016. Reduced expression of IA channels is associated with post-ischemic seizures.. Epilepsy Research, 40-48.
26400949	1 : 1000	Simkin, D., et al. 2015. Aging-related hyperexcitability in CA3 pyramidal neurons is mediated by enhanced A-type K+ channel function and expression. . Journal of Neuroscience, 13206-13218.
23066017	1:2000	Yao, J.J., et al. 2012. Neuritin activates insulin receptor pathway to up-regulate Kv4.2-mediated transient outward K+ current in rat cerebellar granule neurons.. The Journal of Biological Chemistry, 41534-41545.
23066017	1:2000	Yao, J.J., et al. 2012. Neuritin activates insulin receptor pathway to up-regulate Kv4.2-mediated transient outward K+ current in rat cerebellar granule neurons.. The Journal of Biological Chemistry, 41534-41545.
22180649	not listed	Zhang, M., et al. 2012. KCNE2 protein is more abundant in ventricles than in atria and can accelerate hERG protein degradation in a phosphorylation-dependent manner.. American Journal of Physiology-Heart and Circulatory Physiology, H910-H922.
22160437	not listed	Marshall, G.E., et al. 2011. Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade.. Pflügers Archiv: European Journal of Physiology, 537-548.
20849929	1:1000	Dabrowska, J., et al. 2010. Expression and distribution of Kv4 potassium channel subunits and potassium channel interacting proteins in subpopulations of interneurons in the basolateral amygdala.. Neuroscience, 721-33.
19372218	not listed	Jiang, M., et al. 2009. Dynamic partnership between KCNQ1 and KCNE1 and influence on cardiac IKs current amplitude by KCNE2.. The Journal of Biological Chemistry, 16452-16462.
18458082	not listed	Schwenk, J., et al. 2008. NMR analysis of KChIP4a reveals structural basis for control of surface expression of Kv4 channel complexes.. The Journal of Biological Chemistry, 18937-18946.

Additional Publications: Unspecified
PMID	Publication
26121254	Cheng, C.F., et al. 2015. Nerve growth factor-induced synapse-like structures in contralateral sensory ganglia contribute to chronic mirror-image pain.. Pain, 2295-2309.
25447080	Gillet, L., et al. 2015. Cardiac-specific ablation of synapse-associated protein SAP97 in mice decreases potassium currents but not sodium current. Heart Rhythm, 181-192.
25043828	Lei, Z., et al. 2014. Reduced expression of IA channels is associated with postischemic seizures in hyperglycemic rats.. Journal of Neuroscience Research, 1775-1784.
24723263	Dufour, M.A., et al. 2014. Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development.. Journal of Neuroscience Research, 981-999.
23963749	Duarri, A., et al. 2013. The L450F [Corrected] mutation in KCND3 brings spinocerebellar ataxia and Brugada syndrome closer together.. NeuroGenetics, 257-258.
23280838	Duarri, A., et al. 2012. Mutations in potassium channel kcnd3 cause spinocerebellar ataxia type 19.. Annals of Neurology, 870-880.
22155597	Hu, D., et al. 2012. A novel rare variant in SCN1Bb linked to Brugada syndrome and SIDS by combined modulation of Nav1. 5 and Kv4. 3 channel currents. . Heart Rhythm, 760-769.
21895522	Lei, Z., et al. 2012. Alterations of A-type potassium channels in hippocampal neurons after traumatic brain injury.. Journal of Neurotrauma, 235-245.
22220205	Sethi, J., et al. 2011. Kv4.2 mediates histamine modulation of preoptic neuron activity and body temperature.. PLoS One, e29134.
21849540	Hourez, R., et al. 2011. Aminopyridines correct early dysfunction and delay neurodegeneration in a mouse model of spinocerebellar ataxia type 1.. Journal of Neuroscience, 11795-11807.
21507952	Cheng, L., et al. 2011. Cortactin is required for N-cadherin regulation of Kv1.5 channel function.. The Journal of Biological Chemistry, 20478-20489.
21440618	Nowak, A., et al. 2011. Kv3.1b and Kv3.3 channel subunit expression in murine spinal dorsal horn GABAergic interneurones.. Journal of Chemical Neuroanatomy, 30-38.
21115483	Schwetz, T.A., et al. 2011. Sialic acids attached to O-glycans modulate voltage-gated potassium channel gating.. The Journal of Biological Chemistry, 4123-4132.
20650612	Bealer, S.L., et al. 2010. Autonomic and cellular mechanisms mediating detrimental cardiac effects of status epilepticus.. Epilepsy Research, 66-73.
20371829	Norris, A.J., et al. 2010. Molecular dissection of I(A) in cortical pyramidal neurons reveals three distinct components encoded by Kv4.2, Kv4.3, and Kv1.4 alpha-subunits.. Journal of Neuroscience, 5092-5101.
20354865	Cotella, D., et al. 2010. Impaired glycosylation blocks DPP10 cell surface expression and alters the electrophysiology of Ito channel complex.. Pflügers Archiv: European Journal of Physiology, 87-97.
19668710	Phuket, T.R., et al. 2009. Kv4 Channels Underlie the Subthreshold-Operating A-type K-current in Nociceptive Dorsal Root Ganglion Neurons.. Frontiers in Molecular Neuroscience, 3.
19344330	Eyre, M.D., et al. 2009. Molecular diversity of deep short-axon cells of the rat main olfactory bulb.. European Journal of Neuroscience, 1397-1407.
18978958	Clark, B.D., et al. 2008. DPP6 Localization in Brain Supports Function as a Kv4 Channel Associated Protein.. Frontiers in Molecular Neuroscience, 8.
18276729	Amarillo, Y., et al. 2008. Ternary Kv4.2 channels recapitulate voltage-dependent inactivation kinetics of A-type K+ channels in cerebellar granule neurons.. The Journal of Physiology, 2093-2106.
17855600	Chien, L.Y., et al. 2007. Reduced expression of A-type potassium channels in primary sensory neurons induces mechanical hypersensitivity.. Journal of Neuroscience, 9855-9865.
17314290	Bourdeau, M.L., et al. 2007. Kv4.3-mediated A-type K+ currents underlie rhythmic activity in hippocampal interneurons.. Journal of Neuroscience, 1942-1953.

Anti-Kv4.3 K+ Channel Antibody

Cat# 75-017

Contatta il distributore locale :

Marca : Antibodies Incorporated

Contatta il distributore locale :

SKU: 75-017

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Kv4.3 K+ Channel Antibody

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